过表达TAZ促进口腔舌鳞癌细胞增殖迁移和侵袭的作用机制研究  

Promotion of Overexpressing TAZ on Proliferation, Migration, Invasion of Tongue Squamous Carcinoma and the Molecular Mechanism

作　　者：李傲楠 田慧 顾客 陈希彦 王琪 文勇 LI Ao-nan;TIAN Hui;GU Ke;CHEN Xi-yan;WANG Qi;WEN Yong(Shandong Provincial Key Laboratory of Oral Tissue Regeneration,Jinan 250100;School of Stomatology,Shandong University,Jinan 250012,Shandong Province,China)

机构地区：[1]山东省口腔组织再生重点实验室,山东济南250100 [2]山东大学口腔医学院种植科,山东济南250012

出　　处：《口腔颌面外科杂志》2018年第4期192-197,共6页Journal of Oral and Maxillofacial Surgery

基　　金：山东省自然基金(ZR2018WH08);山东省医药卫生科技发展计划项目(2017WS111);山东省博士后创新基金项目专项基金(201703071)

摘　　要：目的:探究过表达Tafazzin(TAZ)对口腔舌鳞癌CAL-27、SCC-15细胞增殖迁移侵袭的影响及其作用机制。方法:利用成功过表达TAZ的慢病毒载体转染CAL-27、SCC-15细胞,分为过表达TAZ组(overexpression TAZ,OE TAZ)和对照组(negative control,NC)。用CCK-8实验检测细胞增殖;用划痕实验检测细胞的迁移;用Transwell实验检测细胞侵袭。相关基因的mRNA水平和蛋白水平分别利用实时荧光定量PCR(qRT-PCR)和Western blot检测。结果:qRT-PCR和Western blot结果显示,过表达TAZ病毒转染成功;过表达组较对照组增殖升高,迁移侵袭明显增强;增殖相关蛋白p-Erk,p-Akt表达量升高,上皮间充质转化(epithelial-mesenchymal transition,EMT)相关蛋白E-钙黏连蛋白(E-Cadherin)降低,波形蛋白(Vimentin)升高。结论:过表达TAZ可能通过影响p-Erk、p-Akt、E-Cadherin、Vimentin的表达促进口腔舌鳞癌CAL-27、SCC-15细胞的增殖迁移和侵袭。Objective:To investigate the effects of overexpressing TAZ on proliferation,migration and invasion of CAL-27、SCC-15 cells and the underlying molecular mechanism.Methods:Tongue squamous carcinoma cells(CAL-27、SCC-15)were used in this study.Lentivirus packaging cells were transfected with LV5-homo-TAZ and LV5-NC,and divided cells into overexpression TAZ(OE TAZ)group and negative control(NC)group.Cell proliferation was determined using CCK-8 assays.Cell migration was measured by scracth assay and cell invasion was measured by transwell assay.The mRNA and related protein levels were detected by qRT-PCR and western blot assays.Results:Western Blot and qRT-PCR analysis revealed successful TAZ overexpression.In overexpression TAZ group,the proliferation rate increased,and cell migration and invasion increased significantly compared to NC group.Proliferation-related proteins increased,such as p-Erk and p-Akt.Epithelial-mesenchymal transition(EMT)-related protein as E-Cadherin decreased while Vimentin was increased.Conclusion:Overexpression TAZ might promote proliferation,migration and invasion by affecting the protein expressions of p-Erk、p-Akt、E-Cadherin、Vimentin in tongue squamous carcinoma CAL-27、SCC-15 cells.

关 键 词：Tafazzin(TAZ) 口腔鳞癌 细胞增殖 细胞迁移 细胞侵袭 

分 类 号：R782[医药卫生—口腔医学]