过表达TGF-β1可抑制人宫颈癌细胞系C-33A增殖和促进凋亡  

Overexpression of TGF-β1 inhibits proliferation and promotes apoptosis of human cervical cancer cell line C-33A

作　　者：脱勋元[1] 王琰[1] 党云[1] 脱淑梅[1] 蔡炳昕 龙丽霞[1] TUO Xun-yuan;WANG Yan;DANG Yun;TUO Shu-mei;CAI Bing-xin;LONG Li-xia(Gansu Province Maternal and Child Health Hospital,Lanzhou 730050,China)

机构地区：[1]甘肃省妇幼保健院

出　　处：《基础医学与临床》2019年第12期1704-1708,共5页Basic and Clinical Medicine

基　　金：甘肃省科技厅国际合作专项(1604WKCA015)

摘　　要：目的研究TGF-β1基因转染对人宫颈癌细胞系(C-33A)增殖和凋亡的影响。方法取增殖状态良好的宫颈癌细胞C-33A,用基因转染的方法建立TGF-β1过表达质粒,用RT-PCR和Western blot法分别检测TGF-β1 mRNA和蛋白的表达及其Bcl-2和Bax的表达;用MTT实验、Transwell小室迁移实验和流式细胞术分别检测细胞的增殖率、迁移率及凋亡率。结果TGF-β1 mRNA和蛋白在宫颈癌C-33A细胞中的表达低于其他细胞(P<0.05);TGF-β1抑制Bcl-2的表达(P<0.05)、促进Bax的表达(P<0.05);过表达TGF-β1细胞的增殖与迁移速度低于其他细胞(P<0.05),但凋亡速度高于其他细胞(P<0.05),且这种降低与升高均呈时间依赖性。结论过表达的TGF-β1可明显抑制宫颈癌细胞的增殖和促进凋亡。Objective To study the effect of TGF-β1 gene transfection on proliferation and apoptosis of human cervical cancer cells in vitro.Methods Overexpression plasmid of TGF-β1 was established by gene transfection in C-33A cell line with well proliferation status.The expression of TGF-β1 mRNA and protein,Bcl-2 and Bax were detected by RT-PCR and Western blot.Cell proliferation rate,migration rate and apoptosis were examined by MTT assay,Transwell assay and flow cytometry respectively.Results The expression of TGF-β1 mRNA and protein in cervical cancer cells was lower than that of other cells(P<0.05).TGF-β1 inhibited the expression of Bcl-2(P<0.05)and promoted of the expression of Bax(P<0.05).The rate of proliferation and migration of overexpressed TGF-β1 cells were lower than that of other cells(P<0.05),but the rate of apoptosis was higher than that of other cells(P<0.05).The decrease and increase were time dependence.Conclusions Overexpression TGF-β1 can significantly inhibit the proliferation and promote apoptosis of cervical cancer cells.

关 键 词：TGF-Β1 增殖 迁移 凋亡 宫颈癌细胞 

分 类 号：R363.14[医药卫生—病理学]