黔产乌头的二萜生物碱类成分研究  

Study on Diterpenoid Alkaloids in Aconitum carmichaeli from Guizhou Province

作　　者：尹田鹏 罗智慧 王敏 汪泽 YIN Tianpeng;LUO Zhihui;WANG Min;WANG Ze(Zhuhai Campus of Zunyi Medical University&Zhuhai Key Laboratory of Fundamental and Applied Research in Traditional Chinese Medicine,Guangdong Zhuhai 519041,China)

机构地区：[1]遵义医科大学珠海校区/珠海市中药基础及应用研究重点实验室

出　　处：《中国药房》2019年第22期3096-3100,共5页China Pharmacy

基　　金：国家自然科学基金资助项目（No.31860095）;贵州省科技计划项目（No.黔科合基础[2018]1193）;国家级大学生创新创业训练计划项目（No.201710661022）

摘　　要：目的:研究贵州地区产乌头中的二萜生物碱类成分,为进一步丰富乌头属植物的化学成分以及开发利用该药用植物提供参考。方法:取贵州产乌头块根,采用酸提碱沉法提取总生物碱浸膏,并采用硅胶柱层析对总生物碱浸膏进行分离纯化,获得二萜生物碱类成分。结合核磁共振和高分辨质谱分析技术对所获化合物进行结构鉴定。结果与结论:从贵州产乌头中分离鉴定出15个二萜类生物碱,分别为12-epi-15-O-acetyl-17-benzoyl-16-hydroxy-16,17-dihydronapelline(Ⅰ)、宋果灵(Ⅱ)、Songoramine(Ⅲ)、10-羟基乌头碱(Ⅳ)、次乌头碱(Ⅴ)、去氧乌头碱(Ⅵ)、乌头碱(Ⅶ)、新乌头碱(Ⅷ)、14-benzoyltalasamine(Ⅸ)、塔拉萨敏(Ⅹ)、尼奥宁(Ⅺ)、6-表查斯曼宁(Ⅻ)、异塔拉定(ⅩⅢ)、萨卡可尼亭(ⅩⅣ)和多根乌头碱(ⅩⅤ)。其中化合物Ⅸ、Ⅻ均为首次从乌头中分离得到。OBJECTIVE:To study the diterpenoid alkaloids in Aconitum carmichaeli from Guizhou province,and to provide reference for the further enrichment of chemical constituents of plants from the genus Aconitum and the development and utilization of A. carmichaeli from Guizhou province. METHODS:The root tuber of A. carmichaeli from Guizhou province was collected. The total alkaloids of A. carmichaeli roots were extracted by acid extraction and alkali precipitation method. The diterpenoid alkaloids were obtained by isolating and purifying total alkaloids extract with silica gel column chromatography. The structures of the isolated compounds were elucidated on the basis of NMR and MS analysis. RESULTS & CONCLUSIONS:Fifteen diterpenoid alkaloids,including 12-epi-15-O-acetyl-17-benzoyl-16-hydroxy-16,17-dihydronapelline(Ⅰ),songorine(Ⅱ),songoramine(Ⅲ),10-aconifine(Ⅳ),hypaconitine(Ⅴ),deoxyaconitine(Ⅵ),aconitine(Ⅶ),mesaconitine(Ⅷ),14-benzoyltalasamine(Ⅸ),talatisamine(Ⅹ),neoline(Ⅺ),6-epi-chasmanine(Ⅻ),isotalatizidine(ⅩⅢ),sachaconitine(ⅩⅣ)and kalakoline(ⅩⅤ)were identified form A. carmichaeli roots from Guizhou Province. Compounds Ⅸ and Ⅻ were isolated from this specie for the first time.

关 键 词：乌头 成分 二萜生物碱 核磁共振 质谱 鉴定 

分 类 号：R284.1[医药卫生—中药学]