联合检测P504s和Claudin-4在前列腺癌中的表达及其在预后评估中的价值  

Expression of P504s and claudin-4 in prostate cancer and prognostic value of their combined detection

作　　者：毕罡[1] 毛成毅[2] 江军[1] 张尧[1] 张军[1] 龙吟 BI Gang;MAO Chengyi;JIANG Jun;ZHANG Yao;ZHANG Jun;LONG Yin(Department of Urology,Daping Hospital,Army Medical University(Third Military Medical University),Chongqing,400042,China;Department of Pathology,Daping Hospital,Army Medical University(Third Military Medical University),Chongqing,400042,China)

机构地区：[1]陆军军医大学(第三军医大学)大坪医院泌尿外科,重庆400042 [2]陆军军医大学(第三军医大学)大坪医院病理科,重庆400042

出　　处：《第三军医大学学报》2019年第22期2195-2198,共4页Journal of Third Military Medical University

基　　金：重庆市自然科学基金一般项目(CSTC2014jcyjA10115)~~

摘　　要：目的通过联合检测α-甲酰基辅酶A消旋酶(P504s)和紧密连接蛋白-4(Claudin-4)在不同危险因素等级前列腺癌中的表达,探讨P504s和Claudin-4表达在前列腺癌预后评估中的价值。方法选取我院病理组织库2013年1月至2015年7月的前列腺手术或穿刺标本共154例,按照病理诊断分为前列腺癌组84例,年龄52~83(70.9±7.3)岁;前列腺增生组70例,年龄51~85(68.2±7.4)岁。对2组标本组织采用组织芯片技术,构建组织芯片,S-P法检测P504s和Claudin-4蛋白的表达,并与前列腺癌预后进行相关性对比分析。结果 P504s和Claudin-4蛋白在前列腺增生组织中的阳性表达率明显低于前列腺癌组织(P<0.01)。P504s蛋白在不同Gleason评分前列腺癌组间阳性表达率没有明显差异;在不同分期前列腺癌组间的阳性表达率亦未见明显差异。而Claudin-4蛋白在前列腺癌Gleason评分≤6分和≥7分的阳性表达率分别为60.98%和74.42%,两者间比较差异有统计学意义(P<0.05);在前列腺癌分期≤T2a期和≥T2b期的阳性表达率分别为55.26%和78.26%,两者间比较差异有统计学意义(P<0.01)。结论 P504s和Claudin-4蛋白的异常表达可能与前列腺癌的发生和进展相关,Claudin-4在危险等级越高的前列腺癌中的表达越高,联合检测可进一步提高前列腺癌的检出并对预后进行评估。Objective To investigate the expression of P504 s and claudin-4 in prostate cancer tissues and assess the value of their combined detection in prognostic evaluation of prostate cancer. Methods We analyzed a total of 154 surgical specimens or biopsy samples of prostate tissues collected between January, 2013 and July, 2015 in the pathological tissue bank of our hospital. These specimens, which consisted of 84 prostate cancer tissues from patients aged 52 to 83 years(mean 70.9±7.3 years) and 70 benign prostatic hyperplasia tissues from patients aged 51 to 85 years(mean 68.2±7.4 years), were used to construct 60-dot matrix paraffin tissue chips for detection of P504 s and claudin-4 proteins using immunohistochemistry with SP method. The correlation of P504 s and claudin-4 expression with the prognosis of the patients with prostate cancer was analyzed. Results The positive expression rates of P504 s and claudin-4 protein were significantly lower in benign prostatic hyperplasia tissues than in prostate cancer tissues(P<0.01). The tissue microarray data did not reveal significant difference in the positive expression rate of P504 s in prostate cancer tissues with different Gleason scores or in different stages. The positive expression rates of claudin-4 protein was significantly lower in prostate cancer with a Gleason score ≤6 than in that with a score ≥7(60.98% vs 74.42%, P<0.05), and was also lower in prostate cancer in T2 a stage or lower than that in T2 b stage or higher(55.26% vs 78.26%, P<0.01). Conclusion Abnormal expression of P504 s and claudin-4 proteins may be related with the occurrence and progression of prostate cancer, and prostate cancer with a higher level of malignancy expresses higher levels of claudin-4. Combined detection of P504 s and claudin-4 can improve the detection rate of prostate cancer and can be of value in prognostic evaluation of the patients.

关 键 词：前列腺癌 P504S CLAUDIN-4 组织芯片 免疫组织化学 预后 

分 类 号：R730.43[医药卫生—肿瘤] R730.7[医药卫生—临床医学]