趋化因子受体7在食管癌中的表达及临床意义  

Expression and clinical significance of chemokine receptor 7 in esophageal cancer

作　　者：安超伦[1] 褚红军[1] 仲崇俊[2] AN Chao-lun;CHU Hong-jun;ZHONG Chong-jun(Department of Thoracic Surgery,Nantong Third People's Hospital affiliated to Nantong University,Nantong,Jiangsu 226001,China)

机构地区：[1]南通市第三人民医院胸外科,江苏南通226001 [2]南通大学附属南通第一人民医院胸外科,江苏南通226001

出　　处：《中国临床研究》2019年第11期1484-1487,共4页Chinese Journal of Clinical Research

基　　金：江苏省南通市科技计划项目(YYZ15040)~~

摘　　要：目的探讨趋化因子受体(CXCR)7在食管癌中的表达水平及其临床意义。方法对2013年1月至2015年12月在南通第三人民医院及南通第一人民医院收治确诊的56例胸段食管鳞癌患者的临床资料进行回顾性分析。随机选取其手术所获6对癌组织以及癌旁组织,应用Real-time PCR、Western blot检测CXCR7 mRNA以及蛋白表达情况,应用免疫组化方法检测56例癌组织中CXCR7阳性情况,并应用χ^2检验以及Spearman相关分析分析CXCR7阳性与否和患者临床特征的关系。随访截止于2017年12月31日,应用Kaplan-Meier生存分析分析CXCR7阳性与否和患者生存期的关系。结果食管癌组织中CXCR7 mRNA表达水平约为癌旁组织的(1.62±0.08)倍(t=11.370,P<0.01);CXCR7蛋白表达水平约为癌旁组织的(1.50±0.09)倍(t=8.043,P<0.01)。56例患者中42例(75.00%)患者CXCR7阳性;CXCR7阳性率与肿瘤浸润深度、淋巴结转移以及pTNM分期有相关性(P<0.01)。至随访截止时,CXCR7阳性患者中位生存期为38个月(95%CI:14.83~57.17),明显低于CXCR7阴性患者的58个月(95%CI:53.13~61.72,P<0.05)。结论 CXCR7在食管癌中高表达,与肿瘤浸润深度、淋巴结转移以及pTNM分期密切相关,并影响患者预后,可能是食管癌诊治的潜在靶点。Objective To investigate the expression and clinical significance of chemokine receptor(CXCR) 7 in esophageal cancer.Methods The clinical data of 56 patients with thoracic esophageal squamous cell carcinoma diagnosed in Nantong Third People′s Hospital and Nantong First People′s Hospital from January 2013 to December 2015 were retrospectively analyzed.Six pairs of cancer tissues and adjacent tissues were randomly selected.The expression of CXCR7 mRNA and protein were detected by Real-time PCR and Western blot.The positive rate of CXCR7 of cancer tissues in 56 cases was detected by immunohistochemistry.The relationship between CXCR7 positive status and clinical features were analyzed by Chi-square test and Spearman correlation analysis.Follow-up was completed on December 31,2017,and Kaplan-Meier survival analysis was used to analyze the relationship between CXCR7 positive status and patients′ survival.Results The expression of CXCR7 mRNA in esophageal cancer tissues was(1.62±0.08) times than that of adjacent tissues(t=11.370,P<0.01).The expression of CXCR7 protein was(1.50±0.09) times than that of adjacent tissues(t=8.043,P<0.01).CXCR7 was positive in 42 of 56 patients(75.00%).The positive rate of CXCR7 was correlated with tumor invasion depth,lymph node metastasis and pTNM stage(all P<0.01).By the end of follow-up,the median survival time of CXCR7 positive patients was 38 months(95% CI:14.83-57.17),which was significantly lower than that of CXCR7-negative patients(58 months,95% CI:53.13-61.72,P<0.05).Conclusions CXCR7 is highly expressed in esophageal cancer,which is closely related to tumor invasion depth,lymph node metastasis and pTNM staging,and affects the prognosis of patients.It may be a potential target for the diagnosis and treatment of esophageal cancer.

关 键 词：趋化因子受体7 食管癌 侵袭 转移 生存期 

分 类 号：R735.1[医药卫生—肿瘤]