负载不同载药胶束纳米纤维膜的构建及其体外抗炎作用比较研究  

The Fabrication of Polymeric Micelles Loaded Core-Shell Structured Nanofiber Mats and Their Effects on Anti-Inflammation in Vitro

作　　者：刘肖晨 苏俭生[1] LIU Xiao-Chen;SU Jian-Sheng(Department of Prosthodontics,School and Hospital of Stomatology,Tongji University,Shanghai Engineering Research Center of Tooth Restoration and Regeneration,Shanghai 200072,China)

机构地区：[1]同济大学口腔医学院·同济大学附属口腔医院口腔修复教研室上海牙组织修复与再生工程技术研究中心

出　　处：《口腔颌面外科杂志》2018年第3期127-134,共8页Journal of Oral and Maxillofacial Surgery

基　　金：国家自然科学基金（81572114,81371949）;上海市科学技术委员会项目（17140903600）

摘　　要：目的:研究分别负载免疫抑制剂(SP600125、SB203580和PD98059)聚合物胶束的纳米纤维膜,在体外对LPS(脂多糖)诱导的牙周膜细胞(periodontal ligament cells,PDLCs)的抗炎作用的影响,并进行比较。方法:通过薄膜水化法制备载药胶束(SP-PMs、SB-PMs、PD-PMs),并通过静电纺丝技术构建纳米纤维膜,使得免疫抑制剂负载其中。通过细胞学实验检测纳米纤维膜的生物相容性,和其对炎症相关基因及蛋白的表达影响。结果:扫描电镜显示纳米纤维呈线型结构,且纤维直径分布集中,激光共聚焦显示载药胶束负载于纤维中;细胞的增殖不受纳米纤维膜的影响;体外实验表明3种不同的载药纳米纤维膜均能抑制炎症因子的表达,其中负载SP-PMs、SB-PMs的纤维抗炎效果明显好于PD-PMs组。结论:负载SP-PMs或SB-PMs的纳米纤维膜显示出良好的抗炎效果,这为牙周炎的治疗提供了新的展望方向。Objective:The study aimed to assess and make a comparison between nanofiber mats encapsulated with three drug-loaded(SP600125,SB203580 and PD98059)polymeric micelles respectively on the anti-inflammatory effect in vitro.Methods:The drug-loaded micelles(SP-PMs,SB-PMs and PD-PMS)were prepared by the thin-film hydration method,and then encapsulated into nanofibers by using electrospinning technique.Cytotoxicity and the expression levels of inflammation-related genes MMP-2,MMP-9 as well as corresponding proteins were performed by periodontal ligament cells(PDLCs).Results:The TEM images revealed the obtained nanofibers was line shape,and showed a narrow distribution range.The CLSM images verified that the drug-loaded micelles were encapsulated into nanofibers uniformly.The co-culturing of nanofiber mats and PDLCs demonstrated a good biocompatibility.In vitro anti-inflammation test illustrated that three drug-loaded nanofiber mats could both significantly decline the expression of MMP-2 and MMP-9 at gene and protein levels,and the inhibitory effects of SP-PMs and SB-PMs were better than PD-PMs group.Conclusion:The results suggested that SP-PMs or SB-PMs encapsulated nanofiber mat showed great anti-inflammatory effect in vitro.The present article presents an alternative technique that may be considered by periodontologists.

关 键 词：载胶束纳米纤维膜 免疫抑制剂 牙周炎 牙周膜细胞 抗炎作用 

分 类 号：R782.4[医药卫生—口腔医学]