蛋白激酶D2在血管紧张素Ⅱ诱导的病理性心肌肥大中的作用  

Role of protein kinase D2 in angiotensin Ⅱ-induced pathological cardiac hypertrophy

作　　者：魏薇 刘秋子 李琛琛 应开承 李静[3] 李长林 WEI Wei;LIU Qiuzi;LI Chenchen;YING Kaicheng;LI Jing;LI Changlin(College of Public Health,Jining Medical University,Jining,Shandong 272067,China;Institute of Precision Medicine,Jining Medical University,Jining,Shandong 272067,China;Faculty of Pharmacology,Medical College of Nankai University,Tianjin 300071,China)

机构地区：[1]济宁医学院公共卫生学院,山东济宁272067 [2]济宁医学院精准医学研究院,山东济宁272067 [3]南开大学医学院药理学系,天津300071

出　　处：《重庆医学》2019年第22期3781-3784,3789,共5页Chongqing medicine

基　　金：国家自然科学基金项目(81072629)

摘　　要：目的研究蛋白激酶D2(PKD2)在血管紧张素Ⅱ(AngⅡ)诱导的病理性心肌肥大中的作用。方法通过AngⅡ诱导新生大鼠和成年大鼠病理性心肌肥大,应用siRNA干扰抑制心肌细胞PKD2基因表达,探讨PKD2对AngⅡ诱导的病理性心肌肥大形成过程中的调控作用。结果AngⅡ上调新生大鼠和成年大鼠心肌细胞的PKD2基因表达;沉默PKD2阻断AngⅡ诱导病理性心肌肥大基因心房利尿因子(ANF)和β-肌凝蛋白重链(β-MHC)表达上调和细胞表面积增大。结论PKD2参与AngⅡ诱导的病理性心肌肥大,可能是预防干预和逆转心肌肥大的潜在靶点。Objective To investigate the role of protein kinase D2(PKD2)in angiotensinⅡ(AngⅡ)-induced pathological cardiac hypertrophy(PCH).Methods PCH in neonatal and adult rats were induced by AngⅡ.The PKD2 gene expression was inhibited by using siRNA interference.The regulatory effect of KD2 during the formation process of AngⅡ-induced PCH was investigated.Results AngⅡup-regulated PKD2 gene expression in both neonatal and adult rat cardiomyocytes.Silencing PKD2 blocked the up-regulation of AngⅡ-induced PCH gene atrial natriuretic factor(ANF)andβ-myosin heavy chain(β-MHC)expressions and increase of cell surface.Conclusion PKD2 participates in AngⅡ-induced PCH,which may be a potential target point for preventing,interfering and reversing PCH.

关 键 词：心房利尿因子 β-肌凝蛋白重链 心肌细胞 蛋白激酶D2 

分 类 号：R364.3[医药卫生—病理学]