血小板反应素4对肺癌细胞转移及侵袭的影响  

Effect of thrombospondin 4 on metastasis and invasion in lung cancer cells

作　　者：莫正英[1] 王凤琴[1] 艾淑颖[1] MO Zhengying;WANG Fengqin;AI Shuying(Department of Oncology,Taihe Hospital/Affiliated Taihe Hospital,Hubei University of Medicine,Shiyan,Hubei 442000,China)

机构地区：[1]湖北省十堰市太和医院/湖北医药学院附属太和医院肿瘤科

出　　处：《重庆医学》2019年第22期3785-3789,共5页Chongqing medicine

基　　金：国家自然科学基金青年项目(81602391);湖北省科技厅基金项目(2017CFB166);湖北省卫生计生委基金项目(WJ2015Z119)

摘　　要：目的探讨血小板反应素4(TSP-4)对肺癌细胞转移和侵袭的影响。方法用MTT方法检测细胞的增殖,用xCELLigence系统检测细胞的迁移,用3D球体细胞侵袭分析试剂盒检测细胞的侵袭。结果重组TSP-4水平为10μmol/L作用肺癌细胞系后,与0μmol/L组比较,细胞的增殖能力明显增强(P<0.05);在用10μmol/L TSP-4孵育细胞72 h后,细胞的迁移能力显著提高(P<0.05);加入10μmol/L TSP-4第7天时,细胞的侵袭能力显著提高。用siRNA干扰的方法,抑制TSP-4在细胞中的表达后,明显抑制了其侵袭、迁移和增殖的能力,且差异有统计学意义(P<0.05)。结论TSP-4是一种细胞外基质糖蛋白,能促进肺癌的侵袭、转移和增殖。Objective To investigate the effect of thrombospondin-4(TSP-4)on the metastasis and invasion of lung cancer cells.Methods The cellular proliferation was detected by MTT,the xCELLigence assay was used to detect the cellular migration,and the 3D sphere cells invasion assay kit was applied to detect the cellular invasion.Results After recombinant TSP-4 with 10μmol/L level acting on the lung cancer cell line,compared with the 0μmol/L group,the cellular proliferation ability was significantly increased,and the difference was statistically significant(P<0.05).After incubating the cells with 10μmol/L TSP-4,the cellular migration ability was significantly increased(P<0.05).On 7 d after adding 10μmol/L TSP-4,the cellular invasion ability was significantly increased.After inhibiting the expression of TSP-4 in cells by using the siRNA interference method,its invasion,metastasis and proliferation abilities were significantly inhibited,and the differences were statistically significant(P<0.05).Conclusion TSP-4 is an extracellular matrix glycoprotein and can promote the invasion,metastasis and proliferation of lung cancer cells.

关 键 词：血小板反应素4 肺肿瘤 转移 侵袭 

分 类 号：R734.2[医药卫生—肿瘤]