Increased levels of miR-124 in human dental pulp stem cells alter the expression of neural markers  

作　　者：Ameneh Mehri-Ghahfarrokhi Zahra Pourteymourfard-Tabrizi Effat Farrokhi Morteza Hashemzadeh Chaleshtori Mohammad-Saeid Jami 

机构地区：[1]Department of Molecular Medicine,School of Advanced Technologies,Shahrekord University of Medical Sciences,Shahrekord,Iran [2]Cellular and Molecular Research Center,Basic Health Sciences Institute,Shahrekord University of Medical Sciences,Shahrekord,Iran [3]Department of Neurology,David Geffen School of Medicine,University of California Los Angeles(UCLA),Los Angeles,CA,USA

出　　处：《Journal of Otology》2019年第4期121-127,共7页中华耳科学杂志（英文版）

基　　金：supported by deputy of research and technology of Shahrekord University of Medical Sciences(grant number 2178)

摘　　要：Auditory neuropathy is the particular form of deafness in humans which cannot be treated by replacement therapy.Human dental pulp stem cells(hDPSCs)are derived from an ectomesenchymal neural crest cell population.Therefore,they possess a promising capacity for neuronal differentiation and repair.miR-124,a key regulator of neuronal development in the inner ear,is expressed at high levels in auditory and vestibular neurons.Here,we evaluated the possible effect of miR-124 in alteration of neural protein markers expression.Using quantitative reverse transcription-PCR(qRT-PCR)analyses and immunofluorescence staining,we studied the expression patterns of neural progenitor markers(Nestin,NOTCH1,and SOX2)and neural markers(b-tubulin Ⅲ,GATA-3,and peripherin)upon transfection of hDPSCs with miR-124.The qRT-PCR results showed that Nestin was upregulated 6 h post-transfection.In contrast,Nestin expression exhibited a decreasing trend 24 h and 48 h post-transfection.Higher levels of b-tubulin Ⅲ,6 h and 16 h post transfection in RNA level as compared with control cells,were determined in transfected DPSCs.However,b-tubulin-Ⅲ expression decreased 48 h post-transfection.The immunoflourescence results indicated that transfection of hDPSCs with miR-124,only affected Nestin among the studied neural progenitor and neural marker expression in protein level.Auditory neuropathy is the particular form of deafness in humans which cannot be treated by replacement therapy.Human dental pulp stem cells(h DPSCs) are derived from an ectomesenchymal neural crest cell population.Therefore,they possess a promising capacity for neuronal differentiation and repair.mi R-124,a key regulator of neuronal development in the inner ear,is expressed at high levels in auditory and vestibular neurons.Here,we evaluated the possible effect of mi R-124 in alteration of neural protein markers expression.Using quantitative reverse transcription-PCR(q RT-PCR) analyses and immunofluorescence staining,we studied the expression patterns of neural progenitor markers(Nestin,NOTCH1,and SOX2) and neural markers(b-tubulin Ⅲ,GATA-3,and peripherin) upon transfection of h DPSCs with mi R-124.The q RT-PCR results showed that Nestin was upregulated 6 h post-transfection.In contrast,Nestin expression exhibited a decreasing trend 24 h and 48 h post-transfection.Higher levels of b-tubulin Ⅲ,6 h and 16 h post transfection in RNA level as compared with control cells,were determined in transfected DPSCs.However,b-tubulin-Ⅲ expression decreased 48 h post-transfection.The immunoflourescence results indicated that transfection of h DPSCs with mi R-124,only affected Nestin among the studied neural progenitor and neural marker expression in protein level.

关 键 词：SENSORINEURAL HEARING loss miR-124 Spiral GANGLION neurons NESTIN DPSCs 

分 类 号：R73[医药卫生—肿瘤]