大鼠胸腺内注射MBP诱导免疫耐受对手术SBI引起的脑神经功能缺陷和脑水肿作用研究  

Effects of MBP immunization induced by thymus injection on SBI induced brain dysfunction and brain edema in rats

作　　者：姚斌 洪晓雅 尚福泰[2] 惠亮亮[1] 安旭生[1] YAO Bin;HONG Xiaoya;SHANG Futai;HUI Liangliang;AN Xusheng(Department of Critical Care Medicine,Huaian First Hospital Affiliated to Nanjing Medical University,Huaian 223300,Jiangsu,China;Department of Anesthesiology,Huaian First Hospital Affiliated to Nanjing Medical University,Huaian 223300,Jiangsu,China)

机构地区：[1]南京医科大学附属淮安第一医院重症医学科,江苏淮安223300 [2]南京医科大学附属淮安第一医院麻醉科,江苏淮安223300

出　　处：《中南医学科学杂志》2019年第6期593-596,共4页Medical Science Journal of Central South China

摘　　要：为探讨大鼠胸腺内注射髓鞘碱性蛋白(MBP)诱导免疫耐受对手术脑受损(SBI)引起的脑神经功能缺损和脑水肿的影响。选取SD大鼠随机分为假手术组、SBI模型组和MBP处理组,模型组胸腺注射等渗盐水,MBP处理组注射MBP,采用改良神经功能缺陷评分(MNSS)评价各组大鼠神经功能,检测各组大鼠脑水肿体积及血清炎性因子,PCR检测脑组织FasL mRNA表达。结果显示大鼠胸腺内注射MBP诱导免疫耐受,可改善SBI大鼠的脑神经功能缺陷和脑水肿,减轻炎性反应,说明MBP通过诱导免疫耐受,减轻脑受损大鼠的脑神经功能缺陷和脑水肿。To investigate the effect of intrathymic injection of myelin basic protein(MBP)-induced immune tolerance on brain neurological deficits and cerebral edema caused by surgical brain damage(SBI).SD rats were randomly divided into sham operation group,SBI model group and MBP treated group.The SBI model group and the MBP treated group was injected with isotonic saline and MBP,respectively.The neurological function of each group was evaluated by modified neurological deficit score(MNSS).The cerebral edema volume and serum inflammatory factors were detected.The expression of FasL mRNA in brain tissue was detected by PCR.The results showed that intrathymic injection of MBP induced immune tolerance in rats,which may improve brain neurological deficits and brain edema in SBI rats and reduce inflammatory response,hitting MBP may improve brain neurological deficits and brain edema in SBI rats by inducing immune tolerance.

关 键 词：髓鞘碱性蛋白 胸腺 手术脑受损 脑神经功能缺损 脑水肿 大鼠 

分 类 号：R742[医药卫生—神经病学与精神病学]