不同猪种背最长肌中miR-23a、miR-151、miR-299、miR-199a和miR-497的差异表达研究  

Study on Differential Expression of miR-23a, miR-151, miR-299, miR-199a and miR-497 in Longissimus Dorsi of Different Pig Breeds

作　　者：王言 顾以韧[1] 杨雪梅[1] 梁艳[1] 杨跃奎[1] 陶璇[1] 钟志君 陈晓辉[1] 曾凯[1] 何志平[1] 吕学斌[1] WANG Yan;GU Yiren;YANG Xuemei;LIANG Yan;YANG Yuekui;TAO Xuan;ZHONG Zhijun;CHEN Xiaohui;ZENG Kai;HE Zhiping;LV Xuebin(Animal Breeding and Genetics Key Laboratory of Sichuan Province,Sichuan Animal Science Academy,Chengdu 610066,China)

机构地区：[1]四川省畜牧科学研究院动物遗传育种四川省重点实验室

出　　处：《养猪》2019年第6期62-65,共4页Swine Production

基　　金：国家生猪产业技术体系项目（CARS-35）;四川省科研院所基本科研项目（SASA2018A02）;四川省青年科技基金项目（2017JQ0011和2016JQ0041）;四川省科技支撑计划项目（2016NYZ0042、2016NYZ0026和2016NZ0108）;四川省重大科技专项（2017NZDZX0002）;现代农业产业技术体系四川创新团队项目（SCCXTD-001、SCCXTD-005和SCCXTD-009）

摘　　要：为研究miRNA表达量与肉质风味的相关性,试验采用荧光定量PCR技术(Q-PCR)检测了4个猪种背最长肌中miR-23a、miR-151、miR-299、miR-199a和miR-497的差异表达。结果表明,miR-23a、miR-151在藏猪背最长肌中表达最高,miR-299、miR-199a和miR-497在约克夏猪背最长肌中表达最高;相关性分析显示,miR-23a、mi R-151在背最长肌中的表达水平与肌内脂肪含量和硫胺素含量呈正相关,与肌纤维面积、眼肌面积和瘦肉率呈负相关,miR-299、miR-199a和miR-497在背最长肌中的表达水平与肌内脂肪含量和硫胺素含量呈负相关,与肌纤维面积、眼肌面积和瘦肉率呈正相关。综上表明,miR-23a、miR-151对猪肉品质具有正调控作用,miR-299、miR-199a和miR-497对猪胴体品质具有正调控作用。In order to investigate the correlation of miRNA expression with meat flavor phenotype index, the expression levels of miR-23 a, miR-151, miR-299, miR-199 a and miR-497 were measured in longissimus dorsi muscle of 4 pig breeds with quantitative real-time polymerase chain reactions(Q-PCR). The results showed that the expression levels of miR-23 a, miR-151 is highest in longissimus dorsi of Tibetan pigs, the expression levels of miR-299, miR-199 a and miR-497 is highest in longissimus dorsi of Yorkshire pigs;correlation analysis showed that the expression levels of miR-23 a, miR-151 were in positive relation with intramuscular fat contents and thiamine, while were negative relation with myofilament area, eye muscle area and lean meat rate. The expression levels of miR-299, miR-199 a and miR-497 were in negative relation with intramuscular fat contents and thiamine, while were positive relation with myofilament area, eye muscle area and lean meat rate. These results indicated that miR-23 a, miR-151 had positive regulation effect on pork quality, and miR-299, miR-199 a and miR-497 had positive regulation effect on pig carcass quality.

关 键 词：猪 MIRNA Q-PCR 背最长肌 

分 类 号：S828.8[农业科学—畜牧学]