牛磺酸通过MST1-Akt通路诱导人结直肠癌细胞自噬的初步研究  

Preliminary study on autophagy induced by taurine through Mst1-Akt pathway in human colorectal cancer

作　　者：舒婷[1,4] 周慧 李华[3] 万慧芳[3] 刘卓琦[1] 杨晓红[1] 朱伟锋[1] 余乐涵[3] 万福生[1] SHU Ting;ZHOU Hui;LI Hua;WAN Huifang;LIU Zhuoqi;Yang Xiaohong;ZHU Weifeng;YU Lehan;WAN Fusheng(Department of Biochemistry and Molecular Biology,College of Basic Medical Sciences,Nanchang University,Nanchang,330006,China;Department of Nursing,Jiangxi Medical College,Shangrao,Jiangxi,334003,China;The Second Clinical Medical College,Nanchang University,Nanchang,330006,China;Medical Laboratory Education Center,Nanchang University,Nanchang,330006,China)

机构地区：[1]南昌大学基础医学院生物化学与分子生物学教研室,江西南昌330088 [2]南昌大学第二临床医学院,江西南昌330006 [3]南昌大学医学实验教学中心,江西南昌330088 [4]江西医学高等专科学校,江西上饶334003

出　　处：《南昌大学学报（理科版）》2019年第4期370-376,共7页Journal of Nanchang University(Natural Science)

基　　金：国家自然科学基金资助项目(81360032);江西省自然基金资助项目(20161BAB205206)

摘　　要：探讨牛磺酸(Tau)对结直肠癌细胞自噬的影响及初步机制。用牛磺酸处理结直肠癌HT-29和SW480细胞,荧光显微镜(MDC染色)检测细胞自噬体形成,Western blotting分析细胞中自噬及MST1-Akt通路相关蛋白表达。MDC染色显示,40 mmoL处理组和80 mmoL处理组荧光标记自噬颗粒较Control组明显增多,并出现大量自噬体和自噬溶酶体,即40~80 mmoL Tau能诱导自噬小体形成;Western Blotting结果显示,随着Tau浓度增加,HT-29和SW480细胞中MST1,Beclin1和LC3-Ⅱ蛋白表达也呈显著性增加(P<0.01),而HT-29细胞Akt蛋白表达及p-Akt(Ser473)水平则呈显著性降低(P<0.01);且MST1蛋白过表达能增加HT-29细胞Beclin 1和LC3-Ⅱ蛋白表达,降低Akt蛋白表达;Tau可能通过MST1-Akt通路增强结直肠癌细胞中Beclin1和LC3-Ⅱ蛋白表达,诱导自噬。The objective of this paper is to investigate the effects of taurine on autophagy in colorectal cancer cells and its mechanism.HT-29 and SW480 cells of colorectal cancer were treated with taurine at different concentrations.Autophagosome formation was detected by fluorescence microscopy(MDC staining),and the expression of autophagy and related proteins in MST1-Akt pathway was analyzed by Western Blotting.MDC staining results showed that the number of fluorescent-labeled autophagosomes in the 40 mmoL Tau and 80 mmoL Tau groups were significantly higher than that in the Control group,with a large number of autophagosomes and autophagolysosomes.Western blotting results showed that with the increase of Tau concentration,Beclin1 and LC3-Ⅱ protein expressions in HT-29 and SW480 cells of colorectal cancer were also significantly increased(P<0.01),while Akt protein expression and p-Akt(Ser473)levels were significantly decreased with the increase of Tau concentration(P<0.01).Moreover,exogenous overexpression of MST1 protein can increase Beclin 1 and LC3-Ⅱprotein expression in HT-29 cells,and reduce Akt protein expression.Tau can enhance the expression of Beclin1 and LC3-Ⅱprotein in colorectal cancer cells through the MST1-Akt pathway,and induce autophagy.

关 键 词：牛磺酸 自噬 哺乳动物不育系20样激酶1 信号通路 结直肠癌 

分 类 号：Q257[生物学—细胞生物学]