骨碎补治疗骨质疏松的网络药理学研究  

Studying the Mechanism of Drynariae Rhizoma in the Treatment of Osteoporosis by Network Pharmacology

作　　者：韩晓英 齐一璕 齐万里 王旭 HAN Xiaoying;QI Yixun;QI Wanli;WANG Xu(Changchun Hengkang Chinese Medicine Hospital,Changchun 130061 Jilin,China;Changchun University Of Chinese Medicine,Changchun 130117 Jilin,China;The People's Liberation Army Joint Service Support Force 964 Hospital,Changchun 130062 Jilin,China)

机构地区：[1]长春恒康中医医院,吉林长春130061 [2]长春中医药大学,吉林长春130117 [3]中国人民解放军联勤保障部队第九六四医院,吉林长春130062

出　　处：《中药新药与临床药理》2019年第11期1352-1356,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：吉林省省级产业创新专项资金项目（2017C059-1）

摘　　要：目的应用网络药理学方法研究骨碎补治疗骨质疏松的可能机制。方法收集和筛选骨质疏松相关靶蛋白、骨碎补有效活性成分及其对应靶蛋白,利用分子对接验证结合活性,构建并分析骨碎补-活性成分-靶蛋白网络、骨碎补活性成分-靶蛋白-信号通路网络及信号通路中靶蛋白交互网络。结果筛选得到7个活性成分及39个核心靶蛋白,其中5个活性成分与30个靶蛋白之间有较好的结合活性。富集分析该30个靶蛋白分别得到17条与成骨功能相关的信号通路、5条与破骨功能相关的信号通路。信号通路靶蛋白交互分析中靶蛋白丝裂原活化蛋白激酶1(MAPK1)、丝裂原活化蛋白激酶3(MAPK3)、丝裂原活化蛋白激酶14(MAPK14)和糖原合成激酶3β(GSK3β)表现出较高的频率及节点度。结论骨碎补可能通过调节骨代谢平衡中成骨和破骨过程相关信号通路治疗骨质疏松,其中成骨相关信号通路和靶蛋白MAPK1、MAPK3、MAPK14、GSK3β起到主要作用。Objective Network pharmacology method is used to study the potential mechanism of Drynariae rhizoma on treatment of osteoporosis(OP).Methods The osteoporosis-related target proteins,the effective components of osteoclasis supplement and its corresponding targeting proteins were collected and screened,and the binding activity was verified by molecular docking.Drynariae rhizoma-active component-target protein network,Drynariae rhizoma active component-target protein-signal pathway and targeted proteins’interaction network in signaling pathways were constructed and analyzed.Results Seven active ingredients and 39 core target proteins were obtained,among which 5 active ingredients and 30 target proteins showed high binding activities.Enrichment analysis of the 30 target proteins resulted 17 signal pathways related to osteogenesis and 5 signal pathways related to osteoclast.In the interactive analysis of signal pathway target proteins,MAPK1,MAPK3,MAPK14 and GSK3βshowed higher frequency and node degree.Conclusion Drynariae rhizoma may treat osteoporosis by regulating osteoblast and osteoclast related signaling pathways in bone metabolic balance,in which osteogenesis-related signaling pathways and target proteins MAPK1,MAPK3,MAPK14,GSK3βplay major roles.

关 键 词：骨碎补 骨质疏松 网络药理学 分子对接 信号通路 MAPK类靶蛋白 

分 类 号：R285.5[医药卫生—中药学]