RhoA和ROCK蛋白在高脂饮食幼鼠肾小球中的表达及其意义  

Expressions of RhoA and ROCK proteins in glomeruli of young mice fed with high-fat diet and their significances

作　　者：汤淼 艾浩 李晓明 TANG Miao;AI Hao;LI Xiaoming(Department of Histology and Embryology,Jinzhou Mediacal University,Jinzhou 121001,China;Key Laboratory of Follicular Development and Reproductive Health,Jinzhou Mediacal University,Jinzhou 121001,China;Department of Histology and Embryology,GuangdongPharmaceutical University,Guangzhou 510006,China)

机构地区：[1]锦州医科大学组织学与胚胎学教研室,辽宁锦州121001 [2]辽宁省卵泡发育与生殖健康重点实验室,辽宁锦州121001 [3]广东药科大学组织学与胚胎学教研室,广东广州510006

出　　处：《吉林大学学报（医学版）》2019年第6期1315-1319,I0019,共6页Journal of Jilin University：Medicine Edition

基　　金：辽宁省科技厅自然科学基金资助课题(20170540373);辽宁省锦州市科技局科学技术计划项目资助课题(16B1G35)

摘　　要：目的:探讨RhoA和ROCK蛋白在高脂饮食(HFD)C57BL/6J幼鼠肾小球中的表达,阐明RhoA/ROCK通路是否与HFD诱导的肾小球损伤有关。方法:36只雄性C57BL/6J幼鼠随机分为正常对照组和HFD组,每组18只,正常对照组幼鼠给予标准饲料,HFD组幼鼠给予高脂饲料,分别饲养12周。检测幼鼠体质量和血清总胆固醇(TC)及甘油三酯(TG)水平。HE染色、PAS染色和Masson染色观察2组幼鼠肾组织病理形态表现、肾小球系膜和基底膜相对面积以及肾间质纤维组织相对面积,免疫组织化学法检测2组幼鼠肾小球中RhoA和ROCK蛋白表达水平,免疫蛋白印迹法检测2组幼鼠肾小球中RhoA、ROCK和Vimentin蛋白的表达水平。结果:与正常对照组比较,HFD组小鼠体质量、血清TC和TG水平明显升高(P<0.01)。HE染色,HFD组小鼠肾小球内出现明显脂肪变性;PAS染色,HFD组小鼠出现肾小球系膜基质轻度增生;Masson染色,HFD组小鼠肾小球内蓝色胶原纤维染色物质比正常对照组增多;与正常对照组比较,HFD组小鼠肾小球系膜和基底膜相对面积明显增加(P<0.05),肾间质纤维组织相对面积增加(P<0.05);免疫组织化学检测,与正常对照组比较,HFD组幼鼠肾小球中RhoA的ROCK蛋白表达水平升高(P<0.05);免疫蛋白印迹法检测,与正常对照组比较,HFD组幼鼠肾小球中RhoA、ROCK和Vimentin蛋白表达水平升高(P<0.05)。结论:给予HFD的C57BL/6J小鼠肾小球中RhoA和ROCK蛋白高表达可能是诱发肾小球损伤的原因之一。Objective:To investigate the expressions of RhoA and ROCKProteins in glomeruli of the C57BL/6J young mice fed with high-fat diet(HFD),and to elucidate whether the RhoA/ROCKPathway was related to glomerular injury induced by HFD.Methods:Thirty-six male C57BL/6J mice were randomly divided into normal control group and HFD group,and there were 18 mice in each group.The mice in normal control group were given standard diet,and the mice in HFD group were given HFD;they were fed for 12 weeks.The body weights and the serum levels of total cholesterol(TC)and triglyceride(TG)of the young mice in two groups were measured.HE staining,PAS staining and Masson staining were used to observe thePathomorphology of kidney tissue,the relative areas of mesangial membrane and basement membrane and renal interstitial fibrous tissue of the young mice in two groups were detected;immunohistochemical method was used to detect the expression levels of RhoA and ROCK in glomeruli of the young mice in two groups.The expression levels of RhoA,ROCK and vimentinProteins in glomeruli of the young mice in two groups were detected by Western blotting method.Results:Compared with normal control group,the body weight,the serum TC and TG levels of the young mice in HFD group were significantly increased(P<0.01).The HE staining results showed obvious fatty degeneration in the glomeruli of the young mice in HFD group;thePAS staining results showed mildProliferation of glomerular mesangial matrix of the young mice in HFD group;the Masson staining results showed that blue collagen fibers in glomeruli of the mice in HFD group were more abundant than those of the mice in normal control group.Compared with normal control group,the relative area of mesangial membrane and basement membrane of the young mice in HFD group was significantly increased(P<0.05),and the relative area of renal interstitial fibrous tissue was increased(P<0.05).Compared with normal control group,the expression levels of RhoA and ROCKProteins in the glomeruli of the young mice in HFD gro

关 键 词：高脂饮食 肾小球 RHOA蛋白 ROCK蛋白 

分 类 号：R589.2[医药卫生—内分泌]