汉族女性癫痫患者性功能障碍与雌激素受体基因多态性的相关性  

The correlation of sexual dysfunction and estrogen receptor gene polymorphism in Chinese Han women with epilepsy

作　　者：陶丽红 张新江[1] 段作伟[1] 刘一辉 侯红玲 Tao Lihong;Zhang Xinjiang;Duan Zuowei;Liu Yihui;Hou Hongling(Department of Neurology,Affiliated Hospital of Yangzhou University,Yangzhou 225000,China)

机构地区：[1]扬州大学附属医院神经内科,225000

出　　处：《中华行为医学与脑科学杂志》2019年第10期887-892,共6页Chinese Journal of Behavioral Medicine and Brain Science

摘　　要：目的 探讨汉族女性癫痫患者( women with epilepsy,WWE)性功能障碍与雌激素受体(estrogens receptor,ER)基因多态性的相关性.方法 连续收集2015年1月至2017年12月在扬州大学附属医院确诊的112例汉族已婚WWE,120例相匹配的健康汉族已婚女性作为对照组. WWE均接受抗癫痫药物(antiepileptic drugs,AEDs)治疗1年或以上.采用中文版女性性功能指数问卷( fe-male sexual function index,FSFI)调查研究对象的性功能;化学发光法测定血清泌乳素( prolactin, PRL)、卵泡刺激素、黄体生成素、雌二醇、孕酮和睾酮水平;聚合酶链反应-限制性片段长度多态性(polymerase chain reaction-restriction fragment length polymorphisms,PCR-RFLP)方法测定ER基因多态性.采用χ2 检验、独立样本t检验及二分类Logistic回归统计方法,比较两组研究对象性功能、性激素及ER基因型和等位基因频率的差异;分析两组研究对象性功能障碍与性激素及ER基因多态性的相关性.结果 (1)汉族 WWE发生性功能障碍的比例高达70. 5% ( 79/112),而对照组的比例是24. 2%(29/120). WWE 组血清 PRL 水平显著高于对照组[( 315. 40 ± 94. 66) mIU/L, ( 292. 09 ± 76. 65)mIU/L],差异有统计学意义(t=2. 072,P=0. 039). (2) WWE组ER基因PvuⅡ C、AluⅠA等位基因频率显著高于对照组,差异有统计学意义(P=0. 001;P=0. 001). (3)二元Logistic回归分析发现PvuⅡ CC基因型和血清睾酮水平与 WWE 性功能障碍独立相关(OR=6. 074,95% CI:1. 257~29. 352,P=0. 025;OR=0. 412,95%CI:0. 201~0. 842,P=0. 015).结论 ERα基因PvuⅡ多态性可能与汉族WWE性功能障碍的易感性有关,PvuⅡ CC基因型可能是WWE性功能障碍的风险基因型.Objective To investigate the correlation between sexual dysfunction( SD) and estrogen receptor (ER) gene polymorphism in Chinese Han women with epilepsy (WWE). Methods A total of 112 married Chinese Han women with epilepsy were collected from outpatients and inpatients of the Affiliated Hospital of Yangzhou University,and 120 matched healthy women were taken as the control group. WWE were treated with antiepileptic drugs ( AEDs) for one year or more. The Chinese version of female sexual function index ( FSFI) was used to investigate the sexual function of the subjects. The chemiluminescence method was used to detect the sex hormones levels such as prolactin ( PRL),follicle-stimulating hormone (FSH),luteinizing hormone(LH),estradiol(E2),progesterone(P) and testosterone(T). Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to determine the ER gene poly-morphism. Chi-square test,t-test and binary logistic regression were used for statistical analysis. The differ-ences of the sexual function,sex hormone,ER genotype,and allele frequency were compared between the two groups. And the correlation between SD and ER gene polymorphism was analyzed. Results ( 1) The pro-portion of SD in Chinese Han WWE was 70. 5%(79/112),while that was 24. 2%(29/120) in the control group. The serum prolactin (PRL) level in the WWE group was significantly higher than that in the control group (t=2. 072,P=0. 039). (2) The allele frequencies of ER PvuII C and AluI A in the WWE group were significantly higher than those in the control group (P=0. 001;P=0. 001). (3) Binary logistic regression a-nalysis showed that PvuII CC genotype and serum testosterone level were independently correlated with SD (OR= 6. 074, 95% CI: 1. 257-29. 352, P= 0. 025;OR= 0. 412, 95% CI: 0. 201-0. 842, P= 0. 015 ). Conclusion ERα PvuII polymorphism may be related to the susceptibility of SD in Chinese Han WWE,and PvuII CC genotype may be the risk genotype of SD in WWE.

关 键 词：癫痫 女性 性功能障碍 性激素 雌激素受体 

分 类 号：R74[医药卫生—神经病学与精神病学]