离子色谱法测毛竹竹叶单糖和糖醛酸组成  

Monosaccharide and Alduronic Acid Composition of Bamboo Leaves by Ion Chromatography

作　　者：张强 季红福 周燕 范煜[1] 葛青[1] 肖竹钱 毛建卫[1] ZHANG Qiang;JI Hongfu;ZHOU Yan;FAN Yu;GE Qing;XIAO Zhuqian;MAO Jianwei(Zhejiang Provincial Key Lab.for Chem&Bio Processing Technology of Farm Product,Zhejiang Provincial Collaborative Innovation Center of Agricultural Biological Resources Biochemical Manufacturing,School of Biological and Chemical Engineering,Zhejiang University of Science and Technology,Hangzhou 310023,China)

机构地区：[1]浙江省农产品化学与生物加工技术重点实验室浙江省农业生物资源生化制造协同创新中心浙江科技学院生物与化学工程学院

出　　处：《生物质化学工程》2019年第6期33-38,共6页Biomass Chemical Engineering

基　　金：浙江省重点研发项目(2015C02031);浙江省农业生物资源生化制造协同创新中心开放基金(2016KF0035,2016KF0110)

摘　　要：以离子色谱建立了一种简便、快速、准确测定木质纤维基单糖组成的方法。采用Dionex ICS-5000+离子色谱仪,以CarboPAC TM PA10(4 mm×250 mm)阴离子交换柱为分离柱,脉冲安培检测器进行检测,氢氧化钠-乙酸钠进行梯度洗脱,同时检测7种常见单糖和2种糖醛酸,结果表明:在各自线性范围内线性关系良好(R 2≥0.9990),精密度相对标准偏差(RSD)均小于3%,加标回收率98.53%~118.63%,重复性RSD均小于1.16%,检测限为0.01~1 mg/L,定量限为0.05~3 mg/L。对竹叶水解液的单糖组成进行了测定,结果表明:竹叶水解液是由阿拉伯糖、半乳糖、葡萄糖和木糖组成,相应单糖的物质的量比为n(阿拉伯糖)∶n(半乳糖)∶n(葡萄糖)∶n(木糖)=2.9∶0.7∶8.2∶9.7。说明该方法可用于木质纤维基单糖的检测。A simple,rapid and accurate method for the determination of monosaccharide compositions from lignocellulose byionchromatography was established.The HPAEC-PAD method was established to simultaneously detect 7 different monosaccharides and two types of alduronic acid.Dionex ICS-5000+with CarboPAC TM PA10(4 mm×250 mm)column and pulsed amperometric detection was involved in this method.This method could be simultaneously qualitatively and quantitatively analysed by the gradient elution of NaOH and CH 3 COONa.The results showed that the method has good linearity(R 2≥0.9990),high precision(RSD<3%),adequate(98.53%118.63%recovery)and good reproducibility(RSD<1.16%)within the range.The limitions of quantitation and detection were 0.05-3.0 mg/L and 0.01-1 mg/L,respectively.The method was applied to determine the monosaccharide compositions from bamboo leaves hydrolysate.The results showed that bamboo leaves hydrolysate was composed of arabinose,galactose,glucose and xylose.The mole ratio of monosaccharide to n(arabinose)∶n(galactose)∶n(glucose)∶n(xylose)=2.9∶0.7∶8.2∶9.7.This method is successful and can be used for the determination of monosaccharides in lignocellulose.

关 键 词：离子色谱-脉冲安培法 木质纤维素 单糖 糖醛酸 方法学研究 

分 类 号：TQ353.7[化学工程]