TATA结合蛋白在舌粘膜癌变中的过表达  

Upregulation of TBP during Lingual Carcinogenesis

作　　者：刘华[1] 岳万远[1] 骆琦[1] 邵帅 张亮[1] 代晓明[2] LIU Hua;YUE Wan-yuan;LUO Qi;SHAO Shuai;ZHANG Liang;DAI Xiao-ming(Dept.of Oral and Maxillofacial Surgery,The 4th Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650021;Maxillofacial Service of the Department of Plastic Surgery,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032,China)

机构地区：[1]云南省第二人民医院口腔颌面外科,云南昆明650021 [2]昆明医科大学第一附属医院整形外科,云南昆明650032

出　　处：《昆明医科大学学报》2019年第11期30-33,共4页Journal of Kunming Medical University

基　　金：云南省科技厅-昆明医科大学应用基础联合专项基金资助项目[2018FE001(-216)];云南省卫生科技计划项目(2017NS134)

摘　　要：目的探讨TATA结合蛋白(TBP)异常表达与舌粘膜癌变的相关性。方法利用差异表达基因,STEM和Cytoscape plugin cyto Hubba分析舌粘膜癌变过程中0(正常,为方便叙述取代号C),12(癌前病变,代号M),28(鳞癌,代号E)周的动态基因变化。差异表达基因定义为基因在组间变化倍数(FC)≥2,P≤0.05。STEM(short time-series expression miner)阳性表达模式为P<0.05。hub基因取MCC评分大于20。免疫组化法检测TATA结合蛋白(TBP)在人舌癌中的变化,阳性细胞>5%为阳性表达。结果与正常相比,TBP在癌前病变(E vs C)和鳞癌(Mvs C)中表达升高,其表达趋势符合14号模式曲线(P=1.1×10^-123),MCC评分为28。在人舌癌中,TBP表达为阳性(χ^2=11.11,P<0.005)。结论TBP是舌粘膜癌变中的关键基因。Objective To evaluate the relationship between the abnormal expression of TBP and lingual carcinogenesis.Me thods The genetic alterations from lingual mucosa of 0(normal,C),12(precancer,M),28 week(cancer,E)were profiled by differentially expressed gene criteria(FC≥2,P<0.05),short time-series expression miner(STEM)and Cytoscape plugin cyto Hubba.the STEM expression was P<0.05.The MCC valued was set as>20.The expressions of TBP were analyzed by immunohistochemistry in 40 cases of human tongue cancer specimens and 10 cases of normal mucosa respectively.While the number of positive cells were more than 5%,the case was assessed as positive.Re s ults TBP was overexpressed in E vs.C and M vs.C subsets.It was enrolled in the No.14 profile and MCC scored 28 in both subsets.The expressions of TBP were positive in human lingual cancer specimens(χ^2=11.11,P<0.005).Conclus ion TBP is a key gene in lingual carcinogenesis.

关 键 词：舌癌 发病机制 基因 免疫组化 

分 类 号：R739.85[医药卫生—肿瘤]