大黄素对慢性牙周炎模型大鼠牙周膜细胞生物学特性的影响  

The effects of emodin on the biological characteristics of periodontal ligament cells in rats with chronic periodontitis

作　　者：徐梅 牛玉明[2] 梁守建 陈德森 XU Mei;NIU Yuming;LIANG Shoujian;CHEN Desen(Suizhou Hospital,Hubei University of Medicine,441300,China;Taihe Hospital,Hubei University of Medicine;Basic Medical College,Hubei University of Medicine,Shiyan)

机构地区：[1]湖北医药学院附属随州医院(随州市中心医院),441300 [2]十堰市太和医院 [3]湖北医药学院基础医学院

出　　处：《实用口腔医学杂志》2019年第6期790-793,共4页Journal of Practical Stomatology

基　　金：湖北省自然科学基金面上项目(编号:2016CFB567)

摘　　要：目的:观察大黄素对慢性牙周炎模型大鼠牙周膜细胞生物学特性的影响。方法:SD大鼠30只,其中20只构建大鼠慢性牙周炎模型并随机均分为模型组和大黄素组(n=10),10只大鼠设为对照组。大黄素组按2 ml/kg剂量灌胃给予大黄素,模型组和对照组正常饲养对照。于治疗2周后取牙周组织,FCM检测牙周膜细胞增殖周期和细胞活性,Western blotting法检测牙周膜组织过氧化物酶体增殖物激活受体-γ(PPAR-γ)和牙龈组织核因子κB的表达;酶联免疫吸附试验(ELISA)检测牙周膜组织中IL-6、IL-8和IL-1β的浓度。结果:模型组细胞增殖能力和细胞活性均降低,模型组G1/G0和G2/M较对照组明显升高,而S和G2+S明显降低(P<0.05);且模型组PPAR-γ表达降低、而NF-κB、IL-6、IL-8和IL-1β表达均增强(P<0.05)。与模型组比较,大黄素组牙周膜细胞G1/G0和G2/M较模型组明显降低,而S和G2+S明显升高(P<0.05), PPAR-γ高表达、而NF-κB低表达,IL-6、IL-8和IL-1β浓度降低(P<0.05)。结论:大黄素可促进大鼠牙周膜细胞增殖,增加细胞活性,并显著抑制牙周膜细胞炎症因子IL-6、IL-8和IL-1β的表达。Objective: To observe the effects of emodin on the biological characteristics of periodontal ligament cells in rats with chronic periodontitis. Methods: 20 SD rats were used to make chronic periodontitis models and were randomly divided into model group and emodin group(n=10). The other 10 rats were set as the control group. The rats in emodin group were given gavage administration of emodin according to the dose of 2 ml/kg. 2 weeks after treatment, Flow cytometry(FCM) was used to detect the cell cycle distribution of the periodontal ligament cells. Western blotting method was used to detect the peroxisome proliferator activated receptor-γ(PPAR-γ) and nuclear factor kappa B(NF-κB) in gingival tissue. Enzyme linked immunosorbent assay(ELISA) was used to detect the concentration of IL-6, IL-8 and IL-1β in periodontal ligament cells. Results: The G1/G0 and G2/M phase cells in the model group were significantly more than those in the control group, while the S and G2 + S cells decreased significantly(P<0.05). The expression of PPAR-γ in the model group was reduced, NF-κB and the S and G2+S phase cells were all increased, compared with the control group(P<0.05). Compared with the model group, the G1/G0 and G2/M of the periodontal ligament cells in the emodin group were lower than those in the model group(P<0.05), while the S and G2+S phase cells increased(P<0.05), PPAR-γ was highly expressed, and NF-κB was low. Conclusion: Emodin can promote the proliferation of periodontal ligament cells, increase cell activity, and inhibit the expression of inflammatory factors IL-6, IL-8 and IL-1β in periodontal ligament cells.

关 键 词：大黄素 慢性牙周炎 细胞活性 过氧化物酶体增殖物激活受体γ(PPAR-γ) 炎症因子 

分 类 号：R781.4[医药卫生—口腔医学]