HEMA与牙本质胶原的相互作用研究  

A study on the reaction characteristics of HEMA to dentinal collagen

作　　者：孙翔 余凡 许荣宸 李锋 黄鹂 SUN Xiang;YU Fan;XU Rongchen;LI Feng;HUANG Li(Military Stomatology State Key Laboratory,National Center for Clinical Medical Research of Stomatology,Shaanxi Provincial Center for the Study of Oral Bioengineering Technology,Department of Stomatology,Fourth Military Medical University,Xi'an 710032;State Key Laboratory of Military Stomatology,National Center for Clinical Medicine Research of Oral Diseases,Shaanxi Provincial Center for the Research of Oral Bioengineering Technology,Department of Rehabilitation,Stomatology Hospital,Fourth Military Medical University,Xi'an 710032;State Key Laboratory of Military Stomatology,National Center for Clinical Medical Research of Oral Diseases,Shaanxi Provincial Center for the Research of Stomatology and Engineering Technology,Department of the General and Emergency Department of Stomatology,Fourth Military Medical University,Xi'an 710032)

机构地区：[1]军事口腔医学国家重点实验室,口腔疾病国家临床医学研究中心,陕西省口腔生物工程技术研究中心,第四军医大学口腔医院特诊科,西安710032 [2]军事口腔医学国家重点实验室,口腔疾病国家临床医学研究中心,陕西省口腔生物工程技术研究中心,第四军医大学口腔医院修复科,西安710032 [3]军事口腔医学国家重点实验室,口腔疾病国家临床医学研究中心,陕西省口腔生物工程技术研究中心,第四军医大学口腔医院综合和急诊科,西安710032

出　　处：《实用口腔医学杂志》2019年第6期875-878,共4页Journal of Practical Stomatology

基　　金：陕西省社会发展科技攻关项目(编号:2015SF152,2017-SF102)

摘　　要：目的:探讨甲基丙烯酸羟乙酯(HEMA)与牙本质胶原之间的相互作用形式。方法:离体牙磨除釉质暴露浅层牙本质,制备牙本质片。经37%磷酸酸蚀后用HEMA处理,使用傅氏转换红外线光谱分析仪(FTIR)检测处理后的红外图谱。使用脱矿牙本质粉末检测其与HEMA反应前后的干重变化情况。利用MOE软件将HEMA与I型胶原进行分子对接,并进行基本的结合模式分析。结果:FTIR结果表明HEMA与脱矿的牙本质之间可能存在弱的化学结合作用,脱矿牙本质粉末与HEMA反应后干重显著增加(P<0.05)。分子对接结果显示HEMA可以与3种I型胶原分子通过氢键结合,结合能均为负值。结论:HEMA与牙本质胶原可以产生氢键结合作用。Objective: To study the interaction form between HEMA and dentinal collagen. Methods: Superficial enamel was removed to obtain dentin specimens. The dentin specimens were etched by 37% phosphoric acid and processed with HEMA. The characteristic spectra of dentin after HEMA treatment was detected by a Fourier Transform Infrared spectrometer(FTIR). The dry weight change of demineralized dentin powder after HEMA processing was detected. Molecular docking between HEMA and collagen molecular was conducted by MOE software and their binding pattern was analyzed. Results: The FTIR results showed that weak chemical interaction existed between HEMA and the demineralized dentin. The dry weight of demineralized dentin powder increased significantly after HEMA treatment(P<0.05). The results of Molecular docking show that HEMA could bind with 3 types collagen I molecules through hydrogen bonding, and the binding energy was all negative. Conclusion: Some hydrogen bonding may take place between HEMA and the dentinal collagen.

关 键 词：胶原 HEMA 牙本质粘接剂 

分 类 号：R783.1[医药卫生—口腔医学]