光周期对龙眼胚性愈伤组织类黄酮类胡萝卜素含量及合成相关基因的表达影响  

Effects of Photoperiod on the Content of Flavonoid Carotenoids and Expression of Synthesis Related Genes in Embryogenic Callus of Longan

作　　者：朱宁 焦楠 张舒婷[1] 李晓斐 赖钟雄[1] ZHU Ning(Instituce of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002)

机构地区：[1]福建农林大学园艺植物生物工程研究所

出　　处：《园艺与种苗》2019年第11期1-5,共5页Horticulture & Seed

基　　金：国家自然科学基金项目（31572088,31672127）;福建省高原学科建设项目（102/71201801101）;福建农林大学科技创新基金（CXZX2017189,CXZX2018076）

摘　　要：[目的]研究不同光周期对龙眼胚性愈伤组织中类黄酮和类胡萝卜素积累、细胞酶活性的影响,以及对类黄酮和类胡萝卜素合成相关基因表达的影响。[方法]以龙眼松散型胚性愈伤组织为材料,研究全光照24 h/0 h L/D、长光照18 h/6 h L/D、半光照12 h/12 h L/D、短光照6 h/18 h L/D、全黑暗0 h/24 h L/D 5种光周期处理下龙眼胚性愈伤组织中类黄酮和类胡萝卜素积累、细胞保护酶的变化,以及类黄酮和类胡萝卜素合成相关基因的表达。[结果]短光照处理下龙眼胚性愈伤组织中类黄酮的含量和产量最高;全光照处理下龙眼胚性愈伤组织中类胡萝卜素含量和产量最高;不同光周期处理下SOD、POD和H2O2的表达趋势基本一致;PRO含量仅全光照时比CK黑暗时期含量高;PAL含量在短光照时最高,与类黄酮短光照时积累量最高的结果一致;光周期处理下类黄酮合成基因CHI和CHS为"M"字表达模式;DFR和F3H为倒"V"字表达模式;类胡萝卜素合成所用的4个基因表达模式各不相同。[结论]该研究表明光周期对龙眼胚性愈伤组织中类黄酮和类胡萝卜素的积累以及其合成相关基因的表达均有调控作用。[Objective]To study the effects of different photoperiods on the accumulation of flavonoids and carotenoids,on cellular enzyme activities and flavonoids and carotenoid synthesis related genes in embryogenic callus of Dimocarpus longan. [Method]Using loose embryogenic callus of D. longan as material,five kinds of photoperiod treatment on the accumulation of flavonoids and carotenoids in longan embryogenic callus were studied. Photocycle processing was as follows:full light 24 h/0 h L/D,long light18 h/6 h L/D,semi-light 12 h/12 h L/D,short-light 6 h/18 h L/D,total darkness 0 h/24 h L/D.The effects of different photoperiod processing on cytoprotective enzymes changes and expression of genes involved in the synthesis of flavonoids and carotenoids were studied also. [Result]The content and yield of flavonoids in embryogenic calli of longan were the highest in short-light treatment,and the hightest carotenoid was treated with 24 h/0 h L/D.The expression trends of SOD,POD and H2 O2 were consistent result under different photoperiod treatments.The PRO content was only higher in the highest period than in the dark period of the control group.The PAL content was highest at in short light,which was consistent with the highest accumulation of flavonoids at 6 h/18 h L/D. The flavonoid synthesis genes CHI and CHS under the photoperiod treatment were "M" expression patterns,and DFR and F3 H were inverted "V" expression patterns.But the four gene expression patterns used for carotenoid synthesis vary. [Conclusion]This study showed that photoperiod regulates the accumulation of flavonoids and carotenoids accumulated and the expression of its synthesis-related genes in the embryo-genic callus of D. longan.

关 键 词：龙眼胚性愈伤组织 光周期 类黄酮 类胡萝卜素 基因表达 

分 类 号：S667.2[农业科学—果树学] Q78[农业科学—园艺学]