半乳糖凝集素9在不同宫颈病变和不同人乳头瘤病毒型别感染中的表达及意义  

Expression and clinical significance of galectin-9 in different cervical lesions and different types of human papillomavirus infection

作　　者：陈剑[1] 刘艳[1] Chen Jian;Liu Yan(Department of Clinical Laboratory,Yangzhou Maternal and Child Health-Care Hospital Affiliated to Yangzhou University,Yangzhou,Jiangsu 225000,China)

机构地区：[1]扬州大学医学院附属医院扬州市妇幼保健院检验科,225000

出　　处：《中国基层医药》2019年第22期2748-2751,共4页Chinese Journal of Primary Medicine and Pharmacy

摘　　要：目的分析半乳糖凝集素9(Gal-9)在不同宫颈病变中以及不同人乳头瘤病毒(HPV)型别感染中的表达差异及临床意义.方法选取扬州市妇幼保健院2016年5月至2018年4月诊治的宫颈癌患者(CC组)42例、高级别宫颈上皮内瘤变患者(HSIL组)45例、低级别宫颈上皮内瘤变患者(LSIL组)45例及健康体检者(NC组)45例为观察对象,采用酶联免疫吸附试验(ELISA)法检测各组血清Gal-9表达水平,运用基因扩增技术及导流杂交原理对观察对象进行HPV 37种分型检测.结果宫颈病变患者中,≥50岁50例(28.3%),其中CC组32例(76.2%)、HSIL组10例(22.2%)、LSIL组8例(17.8%),CC组年龄与HSIL组、LSIL组差异均有统计学意义(t=7.959、10.440,均P<0.05);宫颈病变患者血清Gal-9水平,CC组(22.27±1.72)ng/L,HSIL组(15.43±2.14)ng/L,LSIL组(15.24±1.89)ng/L,均明显高于NC组的(12.08±2.13)ng/L(t=16.360、18.100、24.440,均P<0.05).宫颈病变患者HPV感染率达84.84%(112/132),CC组HPV16阳性83.3%(35/42),HSIL组以HPV16(28.8%)、HPV33(20.0%)、HPV52(17.7%)、HPV58(13.3%)为主;LSIL组以HPV52(26.7%)、HPV58(24.4%)、HPV16(20.0%)、HPV18(8.9%)为主.所有对象感染率前四位的为HPV16、HPV58、HPV52、HPV33,Gal-9与HPV感染的型别无相关性.结论Gal-9在不同宫颈病变中的表达有明显差异,对HPV高危感染者以及Gal-9水平较高患者进行早期干预早期治疗有助于减少宫颈癌的发生.Objective To analyze the expression differences and clinical significance of galectin 9(Gal-9)in different cervical lesions and human papillomavirus(HPV)type infection.Methods From May 2016 to April 2018,42 cases of cervical cancer(CC group),45 cases of high level cervical intraepithelial neoplasia(HSIL group),45 cases of lower cervical intraepithelial neoplasia(LSIL group)in Yangzhou Maternal and Child Health-Care Hospital were selected,and 45 cases of normal outpatient medical examination in our hospital were selected as the control group(NC group).Enzyme-linked immunosorbent assay(ELISA)method was used to detect the serum level of Gal-9.The gene amplification technique and the principle of diversion hybridization were used to detect the 37 types of HPV.Results Of women with cervical lesions,50 cases(28.3%)were 50 years or more,the CC group had 32 cases(76.2%),HSIL group had 10 cases(22.2%),LSIL group had 8 cases(17.8%),the age of CC group compared with HSIL group and LSIL group had significant differences(t=7.959,10.440,all P<0.01).The serum level of Gal-9 in CC group[(22.27±1.72)ng/L],HSIL group[(15.43±2.14)ng/L],LSIL group[(15.24±1.89)ng/L]was significantly higher than that in NC group[(12.08±2.13)ng/L](t=16.360,18.100,24.440,all P<0.05).The HPV infection rate in patients with cervical lesions reached 84.84%(112/132),the positive rate of HPV16 in CC group was 83.3%(35/42),HSIL group was dominated by HPV16(28.8%),HPV33(20.0%),HPV52(17.7%)and HPV58(13.3%),LSIL group was dominated by HPV52(26.7%),HPV58(24.4%),HPV16(20.0%)and HPV18(8.9%).There was no statistically significant difference in the levels of Gal-9 of HPV16,HPV58,HPV52 and HPV33 patients,indicated that there was no statistically significant correlation between Gal-9 level and the type of HPV infection.Conclusion Early intervention in patients with high HPV infection and Gal-9 levels can help reduce the incidence of cervical cancer.

关 键 词：半乳糖凝集素类 宫颈疾病 宫颈肿瘤 癌 鳞状细胞 宫颈上皮内瘤样病变 人乳头瘤病毒 基因型 基因表达 

分 类 号：R73[医药卫生—肿瘤]