Cloning and Expression of Extremely Heat-Resistant Endo-β-1,4-Glucanase Gene from Thermotoga maritima in Bacillus subtilis  

作　　者：HAO Yarong CHEN Ting ZHANG Xingqun 郝亚荣;陈婷;张兴群(Key Laboratory of Science&Technology of Eco-Textile,Ministry of Education,Shanghai 201620,China;College of Chemistry,Chemical Engineering and Biotechnology,Donghua University,Shanghai 201620,China)

机构地区：[1]Key Laboratory of Science&Technology of Eco-Textile,Ministry of Education,Shanghai 201620,China [2]College of Chemistry,Chemical Engineering and Biotechnology,Donghua University,Shanghai 201620,China

出　　处：《Journal of Donghua University(English Edition)》2019年第5期479-482,共4页东华大学学报（英文版）

基　　金：National Natural Science Foundation of China(Regional Fund)(No.51863020)

摘　　要：Gene encoding endo-β-1,4-glucanase(TM1525)is derived from Thermotoga maritima(T.maritima),which has an open reading frame of 825 bp and encodes a 274 amino acid endo-β-1,4-glucanase.This enzyme has the same high temperature resistance as thermophilic bacteria,which is an ideal property for industrial applications.By molecular biological means,TM1525 was cloned into pHT43 vector and introduced into Bacillus subtilis(B.subtilis)WB800N by electroporation.The results showed that the WB800N expression system was successfully constructed,and extracellular expression of the recombinant gene was achieved.Cellulose hydrolyzed activity of the protein was exhibited.Gene encoding endo-β-1,4-glucanase( TM1525) is derived from Thermotoga maritima( T. maritima),which has an open reading frame of 825 bp and encodes a 274 amino acid endo-β-1,4-glucanase. This enzyme has the same high temperature resistance as thermophilic bacteria,which is an ideal property for industrial applications. By molecular biological means,TM1525 was cloned into p HT43 vector and introduced into Bacillus subtilis( B. subtilis) WB800 N by electroporation. The results showed that the WB800 N expression system was successfully constructed, and extracellular expression of the recombinant gene was achieved. Cellulose hydrolyzed activity of the protein was exhibited.

关 键 词：endo-β-1 4-glucanase pHT43 BACILLUS SUBTILIS WB800N THERMOTOGA maritima 

分 类 号：Q812[生物学—生物工程]