National Natural Science Foundation of China(Regional Fund)(No.51863020)
Gene encoding endo-β-1,4-glucanase(TM1525)is derived from Thermotoga maritima(T.maritima),which has an open reading frame of 825 bp and encodes a 274 amino acid endo-β-1,4-glucanase.This enzyme has the same high tem...
Supported by the National High Technology Research and Development Program of China(No.2013AA102104) and the National Natural Science Foundation of China(Nos.20772046, 21072075).
A novel thermostable β-glucosidase(Tnap0602) with β-galactosidase activity was cloned from Thermotoga naphthophila RUK-10 and overexpressed in Escherichia coli BL21(DE3) with the aid of pET28b(+) vector. The ...
We cloned and expressed a new recombinant β-galactosidase(TN0949) from Thermotoga naphthophila RKU-10 with the pET28a(+) vector system in Escherichia coli BL21(DE3), and determined its catalytic capability to ...