131I标记适配子的优化研究及其稳定性的评价  

Optimization and evaluation of the stability of 131I labeling aptamer

作　　者：许杰华[1] 张桂雄 杨敏[1] 黄文山 Xu Jiehua;Zhang Guixiong;Yang Min;Huang Wenshan(Department of Nuclear Medicine,the Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China)

机构地区：[1]中山大学附属第三医院核医学科

出　　处：《新医学》2019年第12期894-897,共4页Journal of New Medicine

基　　金：国家自然科学基金（81101866）;广东省自然科学基金（2018A030313200)

摘　　要：目的探讨用氯氨T法和Iodogen法进行适配子的131I标记的可行性及标记产物在不同条件下的稳定性。方法用氯氨T法和Iodogen法分别对适配子进行131I的标记,用纸层析法测定2种方法不同反应时间的标记率,测定标记产物在不同条件下的稳定性。结果2种方法在反应时间为1 min时的标记率分别为(94.11±0.30)%和(94.01±0.07)%,比较差异无统计学意义(P>0.05);置于4℃与室温24 h后,标记产物在磷酸盐缓冲液(PBS)、无血清培养基、含10%血清培养基、纯血清中的放射性化学纯度分别为(97.46±0.77)%、(94.76±1.57)%、(85.18±2.27)%、(67.96±1.91)%与(95.78±1.98)%、(56.11±4.06)%、(23.96±3.76)%、(9.45±1.75)%,除PBS介质外,标记产物在4℃条件与室温条件下的放射性化学纯度均有差异(P均<0.001);2种温度条件下标记产物在无血清培养基与含10%血清培养基中的放射性化学纯度均有差异(P均<0.001)。结论氯氨T法和Iodogen法均可高效标记适配子,标记产物在低温及无血清条件中稳定性更好。Objective To investigate the feasibility of labeling aptamer with 131I using chloramine-T or Iodogen methods and evaluate its stability under different conditions.Methods Aptamer was labeled with 131I using the chloramine-T and Iodogen methods.The labeling rate of different reaction times were measured by paper chromatography.The stability of labeled products under different conditions was determined.Results The labeling rates of two methods at 1 min of reaction time were(94.11±0.30)%and(94.01±0.07)%,respectively,there was no significant difference between them(P>0.05).The radiochemical purity of labeled products in phosphate buffer(PBS),serum-free medium,10%serum medium and serum were(97.46±0.77)%,(94.76±1.57)%,(85.18±2.27)%and(67.96±1.91)%at 4℃ for 24 h,(95.78±1.98)%,(56.11±4.06)%,(23.96±3.76)%and(9.45±1.75)%at room temperature for 24 h,respectively.Statistical significance was observed under two temperature conditions in serum-free medium,10%serum medium and serum(all P<0.001).At 4℃ and room temperature,the radiochemical purity of labeled products in the serum-free medium and 10%serum medium significantly differed(both P<0.001).Conclusion Both chloramine-T and Iodogen methods can efficiently label the aptamer,and the labeling products have better stability under low temperature and serum-free conditions.

关 键 词：适配子 氯氨T法 IODOGEN法 131I 稳定性 

分 类 号：R73[医药卫生—肿瘤]