依达拉奉对大鼠海水浸泡开放性颅脑外伤水通道蛋白4表达的影响  

Effects of edaravone on the expression of AQP4 in rats with open craniocerebral injury coupled with seawater immersion

作　　者：杨冰 李明 刘信龙 许峰峰 徐正平 Yang Bing;Li Ming;Liu Xinlong;Xu Fengfeng;Xu Zhengping(Department of Neurosurgery,Naval Special Medical Center,Shanghai 200052,China)

机构地区：[1]海军特色医学中心,上海200052

出　　处：《中华航海医学与高气压医学杂志》2019年第5期391-395,共5页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：南京军区医学科技课题(14zd11)。

摘　　要：目的探讨依达拉奉对大鼠海水浸泡开放性颅脑外伤水通道蛋白4(AQP4)表达的影响.方法采用控制性皮质撞击法致伤大鼠颅脑外伤,致伤后将局部损伤的脑组织浸泡在人工海水或生理盐水中,构建动物模型.依达拉奉干预组浸泡结束后给予依达拉奉10 mg/kg腹腔内注射.颅脑外伤海水浸泡组和依达拉奉干预组每组63只大鼠,对照组21只.观察各组大鼠在浸泡后3、8和24 h时间点病理学变化、脑含水量、血脑屏障开放程度、AQP4蛋白表达水平.结果3 h海水浸泡组AQP4半定量为0.222±0.018,依达拉奉干预组为0.380±0.012,对照组为0.396±0.011;8 h海水浸泡组AQP4表达量为0.228±0.015,依达拉奉干预组为0.162±0.013;24 h海水浸泡组为0.462±0.013,依达拉奉干预组为0.076±0.017.方差分析证实各组之间差异均有统计学意义(P<0.05).结论依达拉奉可以扭转开放性颅脑外伤海水浸泡大鼠浸泡后8~24 h内AQP4升高的趋势,其作用机制可能与清除自由基,减轻血脑屏障损伤有关;依达拉奉引起的AQP4表达水平变化也是其治疗海水浸泡开放性颅脑外伤的重要机制.Objective To investigate the effects of edaravone on the expression of aquaporins 4(AQP4)in rats with open craniocerebral injury coupled with seawater immersion.Methods The trauma brain injury rat model was developed by controlled cortical impact(CCI).Following injury,the regional trauma brain injury tissue was immersed in the artificial seawater or physiological saline.The trauma brain injury+seawater immersion group and the edaravone intervention group were respectively composed of 63 rats,and 21 rats were designated as the control group.The edaravone intervention group received intraperitoneal edaravone injection(10 mg/kg)after immersion.The pathological changes,brain water content,blood brain barrier infiltration and AQP4 expression level were observed at timepoints of 3,8 and 24 hours after immersion.Results The semi-quantitative AQP4 expression of the 3-hour seawater immersion group was 0.222±0.018,that of the edaravone intervention group was 0.380±0.012 and that of the control group was 0.396±0.011.The AQP4 expression of the 8-hour seawater immersion group was 0.228±0.015 and that of the edaravone intervention group was 0.162±0.013.The AQP4 expression of the 24-hour seawater immersion group was 0.462±0.013,and that of the edaravone intervention group was 0.076±0.017.Variance analysis indicated that there were significant differences in the obtained data,when comparisons were made between the groups(P<0.05).Conclusion Edaravone could reverse the increasing trend of AQP4 in rats with open craniocerebral trauma injury coupled with seawater immersion within 8 to 24 hours.Its mechanism might be related to the scavenging of free radicals and alleviation of the damage of blood-brain barrier.Changes in AQP4 expression level induced by edaravone might be also an important mechanism in the treatment of open craniocerebral injury coupled with seawater immersion.

关 键 词：开放性颅脑外伤 海水浸泡 水通道蛋白4 依达拉奉 

分 类 号：R74[医药卫生—神经病学与精神病学]