柞蚕饰腹寄蝇基于线粒体Cytb基因的种群变异及系统进化分析  

Population Variability and Phylogeny of Blepharipa tibialis Based on Mitochondiral Cytb Gene

作　　者：杨瑞生 顾羽健 陈玉波 刘彦群 石生林 姜义仁 秦利 Yang Ruisheng;Gu Yujian;Chen Yubo;Liu Yanqun;Shi Shenglin;Jiang Yiren;Qin Li(College of Bioscience and Biotechnology,Shenyang Agricultural University,Liaoning Engineering and Technology Research Center for Insect Resource,Shenyang 110866,China)

机构地区：[1]沈阳农业大学生物科学技术学院辽宁省昆虫资源工程技术研究中心

出　　处：《蚕业科学》2019年第4期509-516,共8页ACTA SERICOLOGICA SINICA

基　　金：国家茧丝绸发展专项(No.201601);现代农业产业技术体系建设专项(No.CARS-18);辽宁省自然科学基金项目(No.2014027002,2014027002)

摘　　要：柞蚕饰腹寄蝇Blepharipa tibialis是柞蚕主要寄生性害虫。扩增得到柞蚕饰腹寄蝇18个种群、长度为554 bp的线粒体Cytb基因片段,生物信息学分析显示其碱基A、T、C和G的平均含量分别为36.10%、42.60%、12.82%和8.48%,AT含量达78.7%,存在明显的AT使用偏向性。样本两两间遗传距离为0~0.000 30,平均遗传距离为0.000 25。柞蚕饰腹寄蝇98个个体中存在6个单倍型,单倍型多样度(H_d)为0.160,平均核苷酸差异数(K)为0.165,个体之间差异性较小。基于Cytb序列片段构建的NJ进化树显示,柞蚕饰腹寄蝇等32种双翅目昆虫从科的水平上与传统分类系统一致,按与柞蚕饰腹寄蝇的亲缘关系由近及远依次将其归类到寄蝇科、蝇科、花蝇科、麻蝇科和丽蝇科。结果表明,柞蚕饰腹寄蝇种群进化时间较短,但线粒体Cytb基因仍能用于双翅目昆虫科级进化的辅助分析。Blepharipa tibialis is the major parasitic pest of Chinese oak silkworm. In present study,mitochondrial Cytb gene fragment with a length of 554 bp was cloned from 18 populations of B. tibialis. Bioinformatical analysis showed that average content of A,T,C and G was 36. 10%,42. 60%,12. 82% and 8. 48%,respectively,presenting significant bias in AT content( 78. 7%). The pairwise genetic distances between 2 individuals ranged from 0 to 0. 000 30,and the average was 0. 000 25. There were 6 haplotype sites in 98 individuals of B. tibialis. Haplotype diversity( Hd) was 0. 160 and average nucleotide difference( K) was 0. 165,indicating only small differences could be found between individuals. NJ tree based on Cytb gene fragment showed that 32 species of Dipera insects like B. tibialis had consistent evolutionary relationship with the traditional taxonomy at family level. They were clustered into family Tachinidae,Muscidae,Anthomyiidae,Sarcophagidae and Calliphoridae in descending order according to their close relationship with B. tibialis. The study suggests that although the evolutionary process for B. tibialis is not long enough for population differentiation,mitochondrial Cytb gene can still be used for auxiliary phylogeny analysis at family level for Dipera insects.

关 键 词：柞蚕饰腹寄蝇 线粒体Cytb基因 种群变异 系统进化 

分 类 号：S885.1[农业科学—特种经济动物饲养] S884.6[农业科学—畜牧兽医]