DDR2和IFITM1在鼻咽癌中的表达及临床意义  

The expression and clinical significance of DDR2 and IFITM1 in nasopharyngeal carcinoma

作　　者：苏日格[1] 曹小莹 Su Rige;Cao Xiaoying(Department of Otolaryngology,the Affiliated Hospital of Inner Mongolia Medical University y Hohhot 010050,China)

机构地区：[1]内蒙古医科大学附属医院耳鼻咽喉科

出　　处：《中国医师杂志》2019年第11期1667-1671,共5页Journal of Chinese Physician

摘　　要：目的探讨盘状结构域受体2(DDR2)、干扰素诱导跨膜蛋白1(IFITM1)在鼻咽癌患者中的表达及其临床意义。方法选取2014年11月至2015年11月本院收治的鼻咽癌患者65例,所有患者均实施鼻咽活检术,活检术后组织标本石蜡包埋,所有标本均经病理学证实。另选取同期65例病理证实为慢性炎症的鼻炎黏膜标本作为对照组。收集两组患者的临床病理资料,实时荧光定量PCR(qRT-PCR)检测DDR2、IFITM1 mRNA表达水平,免疫组化检测鼻咽癌组织DDR2、IFITM1蛋白表达,Pearson法进行相关性分析,对所有患者进行3年随访,采用Kaplan-Meier法对患者进行3年生存期分析。结果qRT-PCR检测结果显示,鼻咽癌组织DDR2、IFITM1 mRNA相对表达水平均显著高于对照组(P<0.05);免疫组化检测结果显示DDR2、IFITM1在鼻咽癌组织中阳性表达率显著高于对照组(P<0.05);DDR2表达与TNM分期、分化程度、浸润深度有关(P<0.05),IFITM1表达与淋巴结转移、TNM分期、分化程度及浸润深度有关(P<0.05);Pearson相关性分析结果显示DDR2与IFITM1呈正相关(r=0.608,P<0.01);Kaplan-Meier分析结果显示,DDR2阴性表达组3年无进展生存率(41.17%)、总生存率(52.94%)显著高于阳性表达组无进展生存率(16.67%)、总生存率(18.75%)(P<0.05),IFITM1阴性表达组无进展生存率(42.86%)、总生存率(61.90%)显著高于阳性表达组无进展生存率(18.18%)、总生存率(20.45%)(P<0.05)。结论DDR2、IFITM1在鼻咽癌组织中均异常高表达,其可能参与鼻咽癌的发生发展过程,且影响患者的预后。Objective To investigate the expression of discoid domain receptor 2(DDR2)and interferon-induced transmembrane protein 1(IFITM1)in nasopharyngeal carcinoma(NPC)patients and their clinical significance.Methods From November 2014 to November 2015,65 patients with nasopharyngeal carcinoma were selected.All patients underwent nasopharyngeal biopsy.All specimens were embedded in paraffin after biopsy.All specimens were confirmed by pathology.Another 65 rhinitis mucosa specimens with chronic inflammation confirmed by pathology in the same period were selected as control group.The clinical and pathological data of the two groups were collected.The mRNA and protein expression levels of DDR2 and IFITM1 in nasopharyngeal carcinoma tissues were detected by quantitative real-time polymerase chain reaction(qRT-PCR)and immunohistochemistry.The correlation between them was analyzed by Pearson.All patients were followed up for 3 years,and the 3-year survival period of the patients was analyzed by Kaplan-Meier method.Results The results of qRT-PCR showed that the relative expression levels of DDR2 and IFITM1 in nasopharyngeal carcinoma tissues were significantly higher than those in control group(P<0.05).Immunohistochemical results showed that the positive rates of DDR2 and IFITM1 in nasopharyngeal carcinoma were significantly higher than those in control group(P<0.05).The results of expressions of DDR2 and IFITM1 and the clinicopathological analysis of the patients showed that the expression of DDR2 was correlated with TNM stage,differentiation degree and infiltration depth,and IFITM1 was correlated with lymph node metastasis,TNM stage,differentiation degree and infiltration depth(P<0.05).Pearson correlation analysis showed that there was correlation between DDR2 and IFITM1(r=0.608,P<0.01).Kaplan-Meier analysis showed that the progression-free survival rate(41.17%)and total survival rate(52.94%)in DDR2 negative expression group were significantly higher than those in positive expression group(16.67%,18.75%,P<0.05).The progressio

关 键 词：鼻咽肿瘤 盘状结构域受体2 干扰素诱导跨膜蛋白1 

分 类 号：R73[医药卫生—肿瘤]