京大戟醋制前后对斑马鱼胚胎心脏的毒性  

Cardiotoxicity in Zebrafish Embryos of Euphorbiae Pekinensis Radix Before and After Processing with Vinegar

作　　者：曹雨诞[1] 张楷承 姚芳[1] 张丽[1] 丁安伟[1] CAO Yu-dan;ZHANG Kai-cheng;YAO Fang;ZHANG Li;DING An-wei(Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing University of Chinese Medicine,Nanjing 210023,China)

机构地区：[1]南京中医药大学江苏省中药资源产业化过程协同创新中心中药资源产业化与方剂创新药物国家地方联合工程研究中心

出　　处：《中国实验方剂学杂志》2019年第24期73-77,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金青年基金项目(81503250);江苏省研究生科研与实践创新计划项目(KYCX18_1618);江苏省“六大人才高峰”项目(2016-YY-026)

摘　　要：目的:以模式生物斑马鱼胚胎为研究模型,考察京大戟醋制前后不同极性部位对心脏的毒性作用。方法:对受精后12 h发育正常的斑马鱼胚胎分别给予京大戟生品和醋制品的石油醚部位、二氯甲烷部位、乙酸乙酯部位提取物,观察受精后72 h药物对斑马鱼胚胎心脏发育和心脏功能的影响。结果:京大戟醋制前后不同极性部位对斑马鱼胚胎均具有心脏毒性,并呈一定的剂量相关性;其中石油醚部位和二氯甲烷部位的毒性作用较为明显,乙酸乙酯部位的毒性较弱;心脏毒性作用主要表现为心脏发育缓慢、心包水肿、心率降低、心脏细胞出现凋亡现象。与生品相应极性部位相比,相近剂量的京大戟醋制品同一极性部位的心脏毒性都有所下降。结论:京大戟对斑马鱼胚胎具有心脏毒性,醋制后毒性降低,可为进一步阐明京大戟醋制减毒的作用机制研究提供一定的实验依据。Objective:Taking zebrafish embryos as research model,to investigate the toxic effect of different polar fractions of Euphorbiae Pekinensis Radix before and after processing with vinegar on heart.Method:Zebrafish embryos with normal development at 12 h after fertilization were treated with petroleum ether,dichloromethane and ethyl acetate extracts of Euphorbiae Pekinensis Radix before and after processing with vinegar for observation of cardiac development and function at 72 h.Result:Various polar fractions of Euphorbiae Pekinensis Radix before and after processing with vinegar had the cardiotoxicity on zebrafish embryos in a dosedependent manner.In addition,the cardiotoxicity of different polar fractions was followed by petroleum ether,dichloromethane and ethyl acetate.The cardiotoxicity was mainly manifested as slow cardiac development,pericardial edema,decrease of heart rate and apoptosis of cardiac cells.Compared with the corresponding polar fraction of raw products,the cardiotoxicity of the same polar fraction of vinegar-processed products with similar doses decreased.Conclusion:Euphorbiae Pekinensis Radix has cardiotoxicity to zebrafish embryos and the cardiotoxicity is reduced after processing with vinegar,which can provide some experimental basis for further elucidation of the detoxication mechanism of Euphorbiae Pekinensis Radix processed with vinegar.

关 键 词：京大戟 醋制 斑马鱼胚胎 心脏毒性 吖啶橙染色 石油醚部位 二氯甲烷部位 

分 类 号：R22[医药卫生—中医基础理论] R28[医药卫生—中医学]