秦艽配方颗粒制备过程中标准汤剂与其不同方法干燥品质量相关性分析  被引量:4

Quality Relevance of Standard Decoction and Different Drying Methods Products for Gentianae Macrophyllae Radix Granules Based on HPLC Fingerprint

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作  者:郭超[1] 焦淑珍[2] 徐超[1] 郝彩琴[1] 冷晓红[1] GUO Chao;JIAO Shu-zhen;XU Chao;HAO Cai-qin;LENG Xiao-hong(Ningxia Polytechnic,Yinchuan 750001,China;School of Agriculture Ningxia University,Yinchuan 750021,China)

机构地区:[1]宁夏职业技术学院,银川750001 [2]宁夏大学农学院,银川750021

出  处:《中国实验方剂学杂志》2019年第24期118-123,共6页Chinese Journal of Experimental Traditional Medical Formulae

基  金:宁夏自然科学基金项目(NZ17235);宁夏第一批青年科技人才托举工程项目。

摘  要:目的:建立秦艽标准汤剂及其不同方法干燥品的HPLC指纹图谱,对其质量相关性进行分析评价。方法:采用HPLC,Inert Sustain C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-磷酸为流动相进行梯度洗脱,检测波长240 nm,流速0.8 m L·min^-1,柱温40℃。以对照指纹图谱及标准汤剂指纹图谱对秦艽标准汤剂不同方法干燥品从化学成分一致性、共有化学成分含量一致性以及主要化学成分含量和转移率等方面进行质量相关性分析。结果:建立秦艽标准汤剂对照指纹图谱,根据峰匹配数据确定15批秦艽标准汤剂指纹图谱中10个共有峰,从10个共有峰中指认出马钱苷酸,6’-O-β-D-葡萄糖基龙胆苦苷,獐牙菜苦苷,龙胆苦苷,獐牙菜苷5个化学成分。质量相关性分析结果表明,3种不同干燥方法制得干燥品水溶液与秦艽标准汤剂在化学成分种类及数量上一致性良好。但在共有化学成分含量及主要化学成分转移率方面,减压干燥所得干燥品与标准汤剂的质量低于喷雾干燥与冷冻干燥所得干燥品。结论:建立了秦艽标准汤剂-不同方法干燥品HPLC指纹图谱,通过化学成分、共有化学成分含量、主要化学成分含量及转移率3方面的质量相关性分析,全面反映了二者质量相关性情况。建议在秦艽配方颗粒制备的关键干燥工艺中采用喷雾干燥或冷冻干燥工艺,该研究为秦艽配方颗粒的制备工艺及质量控制提供了参考依据。Objective:To establish the fingerprints of standard decoction of Gentianae Macrophyllae Radix-dried products by different methods,and to evaluate the quality correlation.Method:HPLC,InertSustain C18 chromatographic column(4.6 mm×250 mm,5μm),Gradient elution was performed for the mobile phase of acetonitrile-phospho,detection wavelength at 240 nm and flow rate of 0.8 m L·min^-1,and column temperature was 40℃.The quality correlation analysis of different methods for different kinds of Gentianae Macrophyllae Radix standard decoction was carried out from the aspects of chemical composition consistency,common chemical composition consistency,main chemical composition content and transfer rate.Result:The control fingerprint of Gentianae Macrophyllae Radix standard decoction was established.According to the peak matching data,there were 10 common peaks in the fingerprint of 15 batches of Gentianae Macrophyllae Radix standard decoction.Among the 10 common peaks,5 chemical constituents of loganic acid,6’-O-β-D-glucosyl gentiopicroside,swertiamarin,gentiopicroside and swertia glycosides were identified.The results of quality correlation analysis showed that the three different drying methods were consistent with the chemical composition and quantity of Gentianae Macrophyllae Radix standard decoction.But in terms of the content consistency of common chemical components and the transfer rate of main chemical components,the quality correlation between the products obtained from vacuum drying and the standard decoction was lower than that obtained from spray drying and freezedrying.Conclusion:The fingerprint of different method of Gentianae Macrophyllae Radix standard decoction was established.Through the analysis of the mass correlation of chemical composition consistency,common chemical composition content consistency,main chemical composition content and transfer rate,the mass correlation between them was comprehensively reflected.It is suggested that spray drying or freeze drying should be used for the key drying p

关 键 词:秦艽 配方颗粒 标准汤剂 减压干燥 喷雾干燥 冷冻干燥 

分 类 号:R289[医药卫生—方剂学] R284.1[医药卫生—中药学]

 

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