超声辅助皂化提取禾谷镰孢菌中麦角甾醇及其HPLC分析  

Optimization of saponification assisted ultrasonic extraction and HPLC analysis of ergosterol from Fusarium graminearum

作　　者：郭瑞 饶斌[1] 吴琴燕[1,2] 陈宏州 杨红福[1] 周华飞[1] 马跃亭 杨敬辉 庄义庆[1] GUO Rui;RAO Bin;WU Qin-yan;CHEN Hong-zhou;YANG Hong-fu;ZHOU Hua-fei;MA Yue-ting;YANG Jing-hui;ZHUANG Yi-qing(Zhenjiang Institute of Agricultural Science in the Hilly Area of Jiangsu Province,Jurong 212400;Zhenjiang Agricultural Science and Technology Industrial Company,Jurong 212400;Jiangsu University of Science and Technology,Zhenjiang 212003)

机构地区：[1]江苏丘陵地区镇江农业科学研究所,句容212400 [2]镇江市农业科学技术实业公司,句容212400 [3]江苏科技大学,镇江212003

出　　处：《分析试验室》2019年第11期1353-1358,共6页Chinese Journal of Analysis Laboratory

基　　金：句容市社会发展项目(SF2018589878);镇江市农业科技支撑项目(NY2018011)资助

摘　　要：采用超声辅助皂化法提取禾谷镰孢菌中的麦角甾醇,通过单因素和响应面分析,对影响麦角甾醇提取效果的主要因素包括皂化碱含量、提取温度、液固比及超声时间进行优化,并建立其HPLC测定方法。结果表明,禾谷镰孢菌中麦角甾醇超声提取最佳条件为碱含量0.034 g/mL、温度36℃、液固比200 mL/g、超声时间8 min,禾谷镰孢菌中麦角甾醇提取量为0.88 mg/g,与模型预测基本一致。麦角甾醇的线性范围为3.125~200μg/mL,相关系数0.9997,平均回收率在82%以上。Ergosterol in Fusarium graminearum(F.graminearum)was extracted by ultrasonic-assisted saponification.The main factors affecting the extraction efficiency of ergosterol were optimized by single factor and response surface analysis,including the content of saponification alkali,extraction temperature,liquid-solid ratio and ultrasonic time.A method for determination of ergosterol by HPLC was established.Results showed that the optimum extraction conditions of ergosterol were alkali content of 0.034 g/mL,temperature of 36℃,liquid-solid ratio of 200 mL/g,ultrasonic extraction time of8 min,and ergosterol extraction amount of 0.88 mg/g,consistent with the model prediction.The calibration curve was linear in the range of 3.125-200μg/mL with the correlation coefficients over0.9997.The average recoveries were more than 82%.

关 键 词：禾谷镰孢菌 麦角甾醇 超声破碎 皂化 响应面 

分 类 号：O652.6[理学—分析化学]