利多卡因对大鼠SAH后神经细胞凋亡和迟发性脑血管痉挛的保护作用  

Protective effects of lidocaine on delayed cerebral vasospasm and neuronal apoptosis after subarachnoid hemorrhage in rats

作　　者：陈博文 苏飞[1] 冉继朋[2] 吴丽欣 李丽杰[4] 魏海波[5] 陈扬 Chen Bowen;Su Fei;Ran Jipeng;Wu Lixin;Li Lijie;Wei Haibo;Chen Yang(Department of Neurosurgery,The First Central Hospital of Baoding,Baoding 071000;Department of Cardiac Vascular Surgery,The First Central Hospital of Baoding,Baoding 071000;Department of Pharmacy,The First Central Hospital of Baoding,Baoding 071000;Department of Neurology,The First Central Hospital of Baoding,Baoding 071000;Department of Pathology,Baoding Maternal and Child Health Hospital,Baoding 071000;Department of Neurosurgery,The Affiliated Hospital of North China University of Science and Technology,Tangshan 063000,China)

机构地区：[1]保定市第一中心医院神经外科,保定071000 [2]保定市第一中心医院心脏血管外科,保定071000 [3]保定市第一中心医院药剂科,保定071000 [4]保定市第一中心医院神经内四科,保定071000 [5]保定市妇幼保健院病理科,保定071000 [6]华北理工大学附属医院神经外二科,唐山063000

出　　处：《神经解剖学杂志》2019年第6期641-645,共5页Chinese Journal of Neuroanatomy

基　　金：保定市科学技术研究与发展指导计划(18ZF114)

摘　　要：目的:探讨利多卡因对大鼠蛛网膜下腔出血(SAH)后神经细胞凋亡和迟发性脑血管痉挛的保护作用。方法:将60只成年大鼠分为假手术组(sham)、SAH组(SAH)和lidocaine处理组(lidocaine)。采用枕大池二次注血法制备动物模型。lidocaine组注射盐酸利多卡因。分别于不同时间灌注后取脑。取用部分基底动脉行苏木精-伊红染色法(HE)观察形态改变和测量基底动脉的管径和管壁的厚度,使用脱氧核糖核酸(DNA)断裂的原位末端标记(TUNEL)法测定海马CA1区神经细胞的凋亡情况。结果:HE染色显示,利多卡因组从第3 d开始内径周长增加,管壁厚度缩小。TUNEL检测显示:SAH组凋亡细胞随时间逐渐增加,而利多卡因组的凋亡细胞逐渐减少。结论:利多卡因能明显减轻大鼠SAH后迟发性的脑血管痉挛及减少海马CA1区神经细胞的凋亡,具有一定的保护作用。Objective: To investigate the protective effects of lidocaine on delayed cerebral vasospasm and neurons in rats with subarachnoid hemorrhage(SAH). Methods: Sixty adult SD rats were divided into 3 groups,including sham group,SAH group and lidocainetreatment group. SAH model was prepared by occipital cisterna secondary blood injection. Thirty minutes after the second injection,rats in sham group and SAH group were injected with saline,while rats in lidocaine group were injected with lidocaine. Brain was taken after perfusion at different time points. Basilar artery(BA) was stained with HE to observe gross morphology and measure tube diameter and wall thickness of BA. Deoxyribonucleotide terminal transferase mediated gap end marker(TUNEL) staining was used to determine apoptotic neurons in the hippocampus CA1 area. Results: HE staining showed that the inner diameter circumference in lidocaine group increased gradually from the 3 rd day,and the tube wall thickness decreased gradually. TUNEL assay showed that apoptotic cells in the SAH group increased gradually with time,while those in the lidocaine group decreased gradually. Conclusion: Lidocaine can significantly reduce the delayed cerebral vasospasm after SAH and reduce the apoptosis of nerve cells in the CA1 region of hippocampus,which has a certain protective effect.

关 键 词：利多卡因 迟发性脑血管痉挛 蛛网膜下腔出血 神经元凋亡 大鼠 

分 类 号：R74[医药卫生—神经病学与精神病学]