长链非编码RNA TUG1对胃癌AGS细胞增殖和凋亡的影响  

Effects of long non-coding RNA TUG1 on the proliferation and apoptosis of gastric cancer AGS cells

作　　者：刘士平[1] 谢俊锋[2] 吴小娟[2] 谢宁生[2] 汤建华[2] 郭广秀[3] LIU Shiping;XIE Junfeng;WU Xiaojuan;XIE Ningsheng;TANG Jianhua;GUO Guangxiu(Department of Geriatrics,People's Hospital of Ganzhou City,Ganzhou 341000,Jiangxi,China;Department of Gastroenterology,People's Hospital of Ganzhou City,Ganzhou 341000,Jiangxi,China;Department of Pathology,People's Hospital of Ganzhou City,Ganzhou 341000,Jiangxi,China)

机构地区：[1]赣州市人民医院老年内科,江西赣州341000 [2]赣州市人民医院消化内科,江西赣州341000 [3]赣州市人民医院病理科,江西赣州341000

出　　处：《中国肿瘤生物治疗杂志》2019年第11期1256-1261,共6页Chinese Journal of Cancer Biotherapy

基　　金：江西省卫计委科技计划资助项目（No.20167202）~~

摘　　要：目的:探究长链非编码RNA牛磺酸上调基因1(long non-coding RNA taurine up-regulated gene 1,lncRNA TUG1)在胃癌组织中的表达及其对胃癌细胞增殖和凋亡的影响。方法:收集2016年3月至2017年12月在江西省赣州市人民医院行胃部手术的病理检查确诊胃癌患者40例,取患者胃部肿瘤组织及其相应癌旁组织(距肿瘤边缘>2 cm处),用qPCR检测胃癌组织标本和胃癌AGS细胞中lncRNA TUG1表达水平。向AGS细胞中转染lncRNA TUG1过表达质粒和TUG siRNA,借助CCK-8、qPCR和流式细胞术检测lncRNA TUG1对胃癌细胞增殖和凋亡的影响。结果:胃癌组织中lncRNA TUG1表达水平显著高于癌旁组织,其与性别、年龄、肿瘤大小、肿瘤浸润程度、淋巴转移、肿瘤分化程度和TNM分期等因素均不相关。过表达lncRNA TUG1显著抑制AGS胃癌细胞中CDKN1A、BAX和Caspase-3表达、减少G1期细胞比例和增加S期细胞比例、提高细胞增殖活力、降低细胞凋亡率;而干扰敲减lncRNA则显著促进细胞中CDKN1A、BAX和Caspase-3表达、增加G1期细胞比例和减少S期细胞比例、降低细胞增殖活力、升高细胞凋亡率。结论:胃癌组织中高表达的lncRNA TUG1促进胃癌细胞增殖而抑制细胞凋亡。Objective: To investigate the expression of lncRNA TUG1(long non-coding RNA taurine up-regulated gene 1) in gastric cancer and its effect on the proliferation and apoptosis of gastric cancer cells. Methods: Surgically resected gastric cancer tissues and corresponding distal normal tissues(>5 cm away from the margin of tumor) of 40 gastric cancer patients from March 2016 to December2017 at Ganzhou People’s Hospital of Jiangxi Province were collected, and qPCR was used to examine the expression of lncRNA TUG1. AGS gastric cancer cells were transfected with lncRNA TUG1 over-expression plasmids and siRNAs, and the effects of lncRNA TUG1 on cell proliferation and apoptosis were assessed by CCK-8, qPCR and Flow cytometry. Results: lncRNA TUG1 expression was significantly increased in gastric cancer tissues as compared to normal tissues;and it was not correlated with gender, age, tumor size, infiltration depth of tumor, lymph node-metastasis, tumor differentiation and TNM staging. TUG1 over-expression significantly suppressed the expressions of CDKN1 A, BAX and Caspase-3 in AGS gastric cancer cells, and decreased G1 phase proportion and apoptosis rate, but increased S phase proportion and cell viability;in contrast, TUG1 siRNA transfection significantly promoted the expressions of CDKN1 A, BAX and Caspase-3, and increased G1 phase proportion and apoptosis rate, but decreased S phase proportion and cell viability. Conclusion: Up-regulated lncRNA TUG1 promotes proliferation and inhibits apoptosis of gastric cancer cells.

关 键 词：胃肿瘤 牛磺酸上调基因1 长链非编码RNA 增殖 凋亡 

分 类 号：R735.2[医药卫生—肿瘤] R730.59[医药卫生—临床医学]