鹰嘴豆(Cicer arietinum)TCP基因家族的生物信息学分析  

Bioinformatics Analysis of TCP Gene Family in Cicer arietinum

作　　者：滕露 倪志勇[1] 于月华[1] Teng Lu;Ni Zhiyong;Yu Yuehua(College of Agriculture,Xinjiang Agricultural University,Urumqi,830052)

机构地区：[1]新疆农业大学农学院

出　　处：《分子植物育种》2019年第22期7313-7319,共7页Molecular Plant Breeding

基　　金：新疆维吾尔自治区自然科学基金面上项目(2016D01A043)资助

摘　　要：TCP蛋白是植物特有转录因子家族之一,参与植物的生长发育调节过程。本研究利用生物信息学方法,通过PlantTFDB database、NCBI、CLUSTALX 2.0、GSDS 2.0、MEME和psRNATarget数据库以及MEGA 5.0等软件,对鹰嘴豆TCP基因家族成员进行分子量、等电点、染色体定位、基因保守域、保守基序、microRNA的靶位点和系统发育进化树的分析。研究表明:TCP基因可被分为ClassⅠ(PCF)和ClassⅡ(CYC/TB1和CIN)两大类,在染色体上的分布较为均匀,具有较保守的螺旋-环-螺旋结构域(Helix loop helix domain,bHLH),含有3个Motif序列,其中每个家族成员共有的是Motif2。microRNA靶位点预测发现,TCP基因的Ⅱ类CIN亚家族中的CarTCP2和CarTCP23受miR319调控。以上结果简要概述了鹰嘴豆TCP基因家族的进化、基因结构及蛋白的生化特性,旨在为今后更好地揭示该基因在鹰嘴豆的生长发育和生理特性中的功能提供理论基础。TCP protein is one of the family-specific transcription factor families involved in the regulation of plant growth and development.This study used bioinformatics methods to perform molecular weight,isoelectric point,chromosomal localization of Cicer arietinum TCP gene family members through PlantTFDB database,NCBI,CLUSTALX 2.0,GSDS 2.0,MEME and psRNATarget databases,and MEGA 5.0 software.Analysis of gene conserved domains,conserved motifs,target sites of microRNAs,and phylogenetic tree.The TCP gene can be divided into two major categories,ClassⅠ(PCF)and ClassⅡ(CYC/TB1 and CIN).The distribution on the chromosome is more uniform,with a more conserved helix-loop-helical domain(bHLH),containing three motif sequences,each of which shares a Motif2.MicroRNA target site prediction revealed that CarTCP2 and CarTCP23 are regulated by miR319 in the classⅡCIN subfamily of the TCP gene.The above results provide a brief overview of the evolution,gene structure and biochemical properties of the TCP gene family of the Cicer arietinum,aiming to provide a theoretical basis for further revealing the function of the gene in the growth,development and physiological characteristics of Cicer arietinum.

关 键 词：TCP基因家族 鹰嘴豆(Cicer arietinum) 生物信息学 

分 类 号：R73[医药卫生—肿瘤]