QCM与光学显微镜共同应用于研究细胞–细胞相互作用对细胞–基质黏附的影响  

Study on Effect of Cell-Cell Interaction on Cell-Substrate Adhesion Using QCM and Optical Microscope

作　　者：黄靓圆 周铁安[1,2] 谭成方 潘炜松[1,2] 李文蔚 HUANG Jingyuan;ZHOU Tiean;TAN Chengfang;PAN Weisong;LI Wenwei(Cell Mechanics and Biosensing Institute,Hunan Agricultural University,Changsha 410128,China;College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]湖南农业大学细胞力学与生物传感研究所,长沙410128 [2]湖南农业大学生物科学技术学院,长沙410128

出　　处：《中国细胞生物学学报》2019年第9期1756-1762,共7页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:21275048、31741045)资助的课题~~

摘　　要：细胞–基质黏附是一个动态而复杂的过程。在细胞培养与伤口愈合等细胞集体迁移过程中,细胞不同密度或细胞–细胞间不同相互作用势必影响细胞–基质间黏附,可有关这种影响的动态研究尚少。石英晶体微天平技术以细胞群为研究对象,能够实时监测细胞与传感元件表面之间的黏附相互作用及细胞黏弹性变化。该文通过培养不同细胞数目以模拟细胞–细胞间不同相互作用,将QCM技术与光学显微镜技术共同应用于研究人脐静脉内皮细胞细胞–细胞相互作用对细胞–基质间黏附的影响。结果显示,细胞–细胞间相互作用对细胞黏附的影响不具有单调性,需分强度范围讨论。当细胞–细胞间相互作用处于较弱范围时,细胞–细胞间相互作用可促进细胞铺展和细胞黏着斑的形成,最终强化细胞黏附,而强劲的细胞–细胞间相互作用会约束细胞铺展,减小黏着斑面积,细胞黏附因此减弱。Cell-substrate adhesion is a dynamic and complex process.During normal cell culture and in the process of collective cell migration such as wound healing,different cell density or cell-cell interactions would affect cell-substrate adhesion,but there are few studies on this dynamic effect.Quartz crystal microbalance(QCM)is an efficient tool that can monitor the adhesion interaction between cells and the sensor surface and the dynamic change in cells’viscoelasticity.In this paper,by culturing different cell numbers to simulate different cell-cell interactions,QCM technology and optical microscopy were used together to study the effect of cell-cell interaction on cell-matrix adhesion in human umbilical vein endothelial cells.The results show that the effect of cell-cell interactions on cell adhesion is not monotonic and should to be discussed in terms of the degrees of cell-cell interactions.When cell-cell interactions are in a weaker range(10000 to 40000 cells),cell-cell interactions promote cell spreading and the formation of focal adhesion,ultimately enhance cell adhesion;while strong cell-cells inter-interactions(60000 to 80000 cells)restrain cell spreading,reduce the area of focal adhesions,and thus weaken cell-substrate adhesion.

关 键 词：石英晶体微天平 光学显微镜 细胞–细胞相互作用 细胞–基质黏附 人脐静脉内皮细胞 

分 类 号：R73[医药卫生—肿瘤]