基于靶向结合的食源性诺如病毒富集与检测研究进展  

Research Progress on Pretreatment and Detection of Norovirus Based on Targeted Binding Functions

作　　者：张乐 吴清平[2] 吴克刚[1] 薛亮[2] 张菊梅[2] ZHANG Le;WU Qingping;WU Kegang;XUE Liang;ZHANG Jumei(Guangdong University of Technology Chemical Engineering and Light Industry,Guangzhou 510070,China;Guangdong Institute of Microbiology,Guangdong Academy of Sciences,State Key Laboratory of Applied Microbiology Southern China,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangdong Open Laboratory of Applied Microbiology,Guangzhou 510070,China)

机构地区：[1]广东工业大学轻工化工学院,广州510070 [2]广东省微生物研究所广东省科学院,省部共建华南应用微生物国家重点实验室,广东省菌种保藏与应用重点实验室,广东省微生物应用新技术公共实验室,广州510070

出　　处：《病毒学报》2019年第6期978-983,共6页Chinese Journal of Virology

基　　金：国家自然科学基金项目(项目号：31701717),题目：衣壳蛋白关键氨基酸突变对食源性诺如病毒GII.17型流行性的影响及分子机制研究;国家重点研发计划项目(项目号：2017YFC1601200),题目：基于组学的食源性致病微生物快速高通量检测技术与装备研发;广州市科技计划项目(项目号：201610010020),题目：广州地区食源性诺如病毒多维度监测体系的构建与分子溯源研究~~

摘　　要：诺如病毒(Noroviruses,NoVs)是全球急性胃肠炎的重要食源性病原。复杂基质的前处理技术一直是食品安全领域中病毒检测研究的重点和难点。除了絮凝沉淀、超速离心、超滤浓缩、电荷膜过滤等常用的非特异性富集技术外,基于分子互作的靶向病毒富集技术已成为了近年来的研究热点。常用的靶向结合种类包括抗原-抗体结合、病毒-受体结合、筛选特异性的核酸适配体、基于噬菌体表面展示技术筛选特异性的多肽、分子印迹等。因此,本文对近年来诺如病毒靶向富集技术的研究进展进行了综述,以期为完善食源性病毒快速检测技术提供参考。Noroviruses are important food-borne pathogens of acute gastroenteritis. Pretreatment technologies in complex substrates are suboptimal for virus detection with regard to food safety. Commonly used non-specific enrichment methods are flocculation,ultracentrifugation,ultrafiltration concentration,and charge membrane filtration. Targeted virus enrichment technologies based on molecular interactions have become"research hotspots"in recent years. Common methods based on virus-targeted binding sites include antigen antibody,viral-receptor,specific nucleic acid aptamers,specific polypeptides screened by phage display,and molecular imprinting. Here, we review recent research progress in targeted enrichment technologies for norovirus detection to provide information for improving their rapid detection.

关 键 词：诺如病毒(NoVs) 靶向结合 免疫磁珠 核酸适配体 受体 噬菌体库 分子印迹 

分 类 号：R155.5[医药卫生—营养与食品卫生学]