胆囊癌患者癌组织及其血清CDO1基因启动子甲基化水平变化及与预后的关系研究  

Changes of promoter methylation of CDO1 gene in cancer tissues and serum of patients with gallbladder cancer and its correlation with prognosis

作　　者：云哲琳[1] 张生彬[1] 张智伟[1] Yun Zhelin;Zhang Shengbin;Zhang Zhiwei(Department of Hepatobiliary Surgery,Inner Mongolia Baotou Iron and Steel Hospital,Baotou 014010,China)

机构地区：[1]内蒙古包钢医院肝胆外科

出　　处：《广西医科大学学报》2019年第11期1792-1796,共5页Journal of Guangxi Medical University

基　　金：内蒙古自治区科学技术厅自然科学基金委面上项目（No.2013MS1102）

摘　　要：目的:研究胆囊癌患者癌组织及血清半胱氨酸双加氧酶-1(CDO1)基因启动子甲基化水平与预后的关系。方法:回顾性分析2016年1月至2017年1月内蒙古包钢医院收治的90例胆囊癌患者的临床资料,依据患者预后情况分为预后良好组(生存时间≥3年,n=40)与预后不良组(生存时间<3年,n=50)。比较两组临床资料(性别、年龄、淋巴结转移情况、组织学类型、TNM分期、肝脏侵犯及胆管侵犯情况)及癌组织和血清CDO1基因启动子甲基化情况,采用单因素及多因素Logistic回归分析法明确胆囊癌患者预后不良的危险因素,并对癌组织及血清CDO1基因启动子完全、部分、无甲基化患者3年生存率进行分析。结果:预后不良组女性、年龄>60岁、淋巴结转移、TNM分期Ⅲ~Ⅳ期、癌组织及血清CDO1基因启动子完全甲基化所占百分比均显著高于预后良好组(P<0.05)。多因素Logistic回归分析显示,上述因素均为胆囊癌患者预后不良的独立危险因素(均P<0.05)。癌组织及血清CDO1基因启动子无甲基化患者3年生存率高于癌组织及血清CDO1基因启动子完全、部分甲基化患者。结论:CDO1基因启动子甲基化水平与胆囊癌预后有关,其可能作为预测胆囊癌患者预后的生物学指标。Objective:To study the changes of promoter methylation of cysteine dioxygenase-1(CDO1)gene in cancer tissues and serum of patients with gallbladder cancer and its correlation with prognosis.Methods:The clinical data of 90 patients with gallbladder cancer in our hospital from January 2016 to January 2017 were retrospectively analyzed.According to the prognosis,the patients were divided into good prognosis group(survival time≥3 years,n=40)and poor prognosis group(survival time<3 years,n=50).The risk factors of the prognosis of patients with gallbladder carcinoma were determined by univariate and multivariate logistic regression analysis.The 3-year survival rate of patients with complete,partial and no methylation of cancer tissue and serum CDO1 gene promoter was analyzed.Results:The percentage of female,>60 years old,lymph node metastasis,TNM stage Ⅲ-Ⅳ,and complete methylation of CDO1 gene promoter in cancer tissue and serum in poor prognosis group were significantly higher than those in good prognosis group(P<0.05).Multivariate logistic regression analysis showed that the above factors were independent risk factors for poor prognosis in patients with gallbladder carcinoma(P<0.05).The 3-year survival rate of patients with unmethylated CDO1 gene promoter in cancer tissues and serum was higher than that of patients with complete and partial methylation of CDO1 gene promoter.Conclusion:The methylated level of CDO1 gene promoter is related to the prognosis of patients with gallbladder carcinoma,which may be used as a biological index to predict the prognosis of patients with gallbladder carcinoma.

关 键 词：胆囊癌 癌组织 血清 半胱氨酸双加氧酶-1基因启动子甲基化 预后 

分 类 号：R735.[医药卫生—肿瘤]