对比剂肾病大鼠肾脏bax及bcl-2表达及羟苯磺酸钙干预研究  

Study on the expression of bax and bcl-2 in kidney of rats with contrast-induced nephropathy and the intervention of Calcium Dobesilate

作　　者：李伟[1,2] 于芬芬 季文萱 何晓艳 潘配强 孙艳 黄俊彦[3] LI Wei;YU Fenfen;JI Wenxuan;HE Xiaoyan;PAN Peiqiang;SUN Yan;Huang Junyan(Medical College,Qingdao University,Shandong Province,Qingdao 266021,China;Department of Nephrology,Pingyi County Hospital of Traditional Chinese Medicine in Linyi City,Shandong Province,Pingyi 273300,China;Department of Nephrology,Qingdao Central Hospital,Shandong Province,Qingdao 266042,China;Department of Nephrology,Qingdao Municipal Hospital,Shandong Province,Qingdao 266011,China)

机构地区：[1]青岛大学医学院,山东青岛266021 [2]山东省临沂市平邑县中医医院肾内科,山东平邑273300 [3]青岛市中心医院肾内科,山东青岛266042 [4]青岛市市立医院肾内科,山东青岛266011

出　　处：《中国医药导报》2019年第32期9-13,I0003,共6页China Medical Herald

基　　金：山东省医药卫生科技发展计划项目（2018WS359）;山东省青岛市民生科技计划项目（16-6-2-19-nsh）

摘　　要：目的研究对比剂肾病(CIN)大鼠肾脏bax及bcl-2的表达情况及羟苯磺酸钙对干预效果。方法将84只大鼠按照随机数字表法分为假手术组(A组)、模型组(B组)和羟苯磺酸钙干预组(C组),每组28只。A组给予生理盐水,B组将INDO、L-NAME、760 g/L泛影葡胺进行注射,C组在B组基础上给予羟苯磺酸钙灌胃。造模后12、24、48、72 h测定血清尿素氮(BUN)和肌酐(Scr),HE染色观察肾病理改变,TUNEL检测肾脏细胞凋亡,免疫组化和Western blot检测肾脏bax及bcl-2的表达。结果B组和C组造模后12、24、48、72 h血清BUN和Scr水平显著高于A组同期,C组造模后12、24、48、72 h血清BUN和Scr水平显著低于B组同期(P<0.05);与造模后12 h比较,B组和C组造模后24、48、72 h血清BUN和Scr水平均显著升高(P<0.05);与造模后24 h比较,B组造模后48 h血清Scr水平显著升高,C组造模后48 h血清BUN和Scr水平均显著升高,C组造模后72 h血清Scr水平显著降低(P<0.05);与造模后48 h比较,B组和C组造模后72 h血清BUN和Scr水平均显著降低(P<0.05)。A组病理改变不显著;B组造模后12 h出现广泛肾小管上皮细胞空泡样变性、部分肾小管坏死,肾间质纤维化;C组肾小管间质病变显著改善。A组大鼠肾细胞无明显凋亡;B组大鼠肾细胞凋亡指数显著高于A组,C组肾细胞凋亡指数显著低于B组,但高于A组(P<0.05)。B组大鼠bax表达水平显著高于A组,C组bax表达水平显著低于B组,但高于A组;B组大鼠bcl-2表达水平显著低于A组,C组bcl-2表达水平显著高于B组,但低于A组(P<0.05)。B组大鼠bax蛋白相对表达量显著高于A组,C组bax蛋白相对表达量显著低于B组,但高于A组;B组大鼠bcl-2蛋白相对表达量显著低于A组,C组bcl-2蛋白相对表达量显著高于B组,但低于A组(P<0.05)。结论细胞凋亡可能参与大鼠CIN的发生、发展,且羟苯磺酸钙可能通过减少bax的表达及增加bcl-2表达减轻肾脏细胞凋亡,发挥保护作用。Objective To investigate the expression of bax and bcl-2 in kidney of rats with contrast-induced nephropathy(CIN)and the effects of Calcium Dobesilate.Methods Eighty-four rats were divided into sham operation group(group A),model group(group B)and Calcium Dobesilate intervention group(group C)according to random number table,28 rats in each group.Group A was given saline,group B was injected with INDO,L-NAME and 760 g/L Diatrizoate,while group C was given Calcium Dobesilate for intragastric administration on the basis of group B.After 12,24,48,72 h model establishment,blood urea nitrogen(BUN)and creatinine(Scr)were measured.Nephropathy was observed by HE staining.Renal cell apoptosis was detected by TUNEL.The expression of bax and bcl-2 were detected by immunohistochemistry and Western blot.Results The levels of BUN and Scr in group B and group C were significantly higher than those in group A at 12,24,48,72 h after the establishment of model,while those in group C were significantly lower than those in group B at 12,24,48,72 h after the establishment of model(P<0.05).Compared with 12 h after modeling,serum BUN and Scr levels in group B and group C were significantly higher at 24,48,72 h after modeling(P<0.05).Compared with 24 h after model establishment,serum Scr level in group B increased significantly at 48 h after model establishment,serum BUN and Scr levels in group C increased significantly at 48 h after model establishment,and serum Scr level in group C decreased significantly at 72 h after model establishment(P<0.05).Compared with 48 h after modeling,serum BUN and Scr levels in group B and group C were significantly lower at 72 h after modeling(P<0.05).In group A,the pathological changes were not significant;in group B,extensive tubular epithelial cell vacuolation,partial tubular necrosis and renal interstitial fibrosis appeared 12 h after modeling;in group C,the renal tubular interstitial lesions were significantly improved.There was no significant apoptosis in group A;the apoptosis index in group B was

关 键 词：对比剂肾病 细胞凋亡 BAX BCL-2 羟苯磺酸钙 

分 类 号：R-332[医药卫生]