炎症性肠病患者CXCR1、IL-17的表达及其意义  

Expression and significance of CXCR1 and IL-17 in patients with inflammatory bowel disease

作　　者：蔡清红 马松炎[1] 钟俊锋[1] CAI Qinghong;MA Songyan;ZHOGN Junfeng(Department of Gastroenterology,Huizhou Third People′s Hospital,Guangdong Province,Huizhou516000,China)

机构地区：[1]广东省惠州市第三人民医院消化内科

出　　处：《中国医药导报》2019年第32期99-102,共4页China Medical Herald

基　　金：广东省自然科学基金项目（2017A030310195）

摘　　要：目的 研究炎症性肠病(IBD)患者CXC趋化因子受体1(CXCR1)、白细胞介素-17(IL-17)的表达水平及其意义。方法 回顾性分析2016年1月~2017年12月惠州市第三人民医院收治的IBD患者80例,其中溃疡性结肠炎(UC)患者70例(UC组),克罗恩病(CD)患者10例(CD组),另选同期在医院进行结肠镜检查的健康志愿者50例纳入对照组。比较各组血清及肠黏膜IL-17及CXCR1 mRNA表达水平,各组肠黏膜CXCR1蛋白表达水平,分析患者血清及肠黏膜IL-17与CXCR1 mRNA表达的相关性。结果 与对照组比较,UC组及CD组血清IL-17mRNA表达水平升高(均P <0.05),但UC组与CD组比较,差异无统计学意义(P> 0.05);各组血清CXCR1 mRNA表达水平比较,差异无统计学意义(P> 0.05);与对照组比较,UC组及CD组肠黏膜IL-17及CXCR1 mRNA表达水平升高(均P <0.05),UC组肠黏膜CXCR1 mRNA表达水平较CD组更高(P <0.05),但两组肠黏膜IL-17表达水平比较,差异无统计学意义(P> 0.05);与对照组比较,UC组及CD组肠黏膜CXCR1蛋白表达水平升高(P <0.05),且UC组较CD组更高,差异有统计学意义(P <0.05)。Spearman法相关性分析发现,患者血清及肠黏膜IL-17 mRNA与CXCR1 mRNA表达呈正相关(P <0.05)。结论 IBD患者CXCR1 m RNA和IL-17 m RNA表达升高,且患者血清及肠黏膜IL-17 mRNA与CXCR1 mRNA表达之间呈弱相关,临床上可考虑监测CXCR1和IL-17水平,从而更好地辅助治疗IBD。Objective To study the expression and significance of CXC chemokine receptor 1(CXCR1)and interleukin-17(IL-17)in patients with inflammatory bowel disease(IBD).Methods The clinical data of 80 patients with IBD admitted to Huizhou Third People′s Hospital from January 2016 to December 2017 was analyzed retrospectively,70 patients with ulcerative colitis(UC group),10 patients with Crohn′s disease(CD group),and 50 healthy volunteers who underwent colonoscopy at the same time were selected as control group.The expression of IL-17 and CXCR1 mRNA in serum and intestinal mucosa of each group was compared,and the protein expression of CXCR1 in intestinal mucosa of each group was analyzed.The correlation between the expression of IL-17 and CXCR1 mRNA in serum and in intestinal mucosa of patients was analyzed.Results Compared with the control group,the expression of IL-17mRNA in serum of UC group and CD group were higher(all P<0.05),but there was no significant difference between UC group and CD group(P>0.05).There was no significant difference in serum CXCR1 mRNA expression between the two groups(P>0.05).Compared with the control group,the expression of IL-17 and CXCR1 mRNA in intestinal mucosa of UC group and CD group was higher(P<0.05).The expression of CXCR1 mRNA in intestinal mucosa of UC group was higher than that of CD group(P<0.05),however,there was no significant difference in IL-17 mRNA expression between the two groups(P>0.05).Compared with the control group,the protein expression of CXCR1 in intestinal mucosa of UC group and CD group was higher,and the protein expression of CXCR1 in UC group was higher than that in CD group(P<0.05).Spearman correlation analysis showed that there was a weak correlation between the expression of IL-17 and CXCR1 in serum and intestinal mucosa(P<0.05).Conclusion The expression levels of CXCR1 mRNA and IL-17 mRNA in serum and intestinal in IBD patients are higher,and there is a positively correlation between the expression of CXCR1 mRNA and IL-17 mRNA in serum and in intestinal mu

关 键 词：炎症性肠病 CXC趋化因子受体1 白细胞介素-17 表达 

分 类 号：R574.1[医药卫生—消化系统]