血清LncRNA-ENST00000420480在铜绿假单胞菌感染中诊断价值  

Clinical value of the serum long noncoding RNA-ENST00000420480 in the diagnosis of Pseudomonas aeruginosa infection

作　　者：李有强 张云燕 刘杨 张轩 蔡雪莹 陈茶 罗燕芬 LI Youqiang;ZHANG Yunyan;LIU Yang;ZHANG Xuan;CAI Xueying;CHEN Cha;LUO Yanfen(Department of Laboratory Medicine,the Affiliated Hexian Memorial Hospital of Southern Medical University,Guangzhou 511400,China)

机构地区：[1]南方医科大学附属何贤纪念医院检验科,广州511400 [2]广州医科大学附属广州市妇女儿童医疗中心口腔科,广州501180 [3]广州中医药大学第二临床医学院,广州510006

出　　处：《实用医学杂志》2019年第21期3370-3373,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号：81601736);广东省科技计划资助项目(编号：2013B021800241,2014A020212267)

摘　　要：目的探讨长链非编码RNAENST00000420480作为铜绿假单胞菌(Pseudomonas aeruginosa,PA)感染诊断标志物的临床价值。方法采用密度梯度离心法分离人外周血单个核细胞,免疫磁珠分选人CD14+单核细胞、hIL?4和hGM?CSF诱导分化为单核细胞衍生树突细胞(monocytes derived dendritic cells,Mo?DCs),分PBS组、LPS组和LPS+3?O?C12?HSL组。实时荧光定量PCR检测lncRNA?ENST00000420480表达量。收集临床PA感染患者血清21例,体检表观健康人血清32例,提取血清RNA,逆转录获得cDNA,荧光定量PCR检测lncRNA?ENST00000420480表达量,受试者工作特征曲线评价血清中lncRNA?ENST00000420480对PA感染的诊断效能,计算约登指数(YI)明确PA感染诊断截点(cut?off)。结果与PBS组相比,LPS下调Mo?DCs中lncRNA?ENST00000420480表达水平。与LPS组相比,3?O?C12?HSL上调Mo?DCs中lncRNA?ENST00000420480表达水平。PA感染组血清中lncRNA?ENST00000420480相对表达量为(3.85±4.18),正常人组血清中lncRNA?ENST00000420480相对表达量为(1.30±1.06),差异有统计学意义(P<0.05)。ROC曲线中AUC为0.708(P=0.01),YI为0.429,诊断截点值为4.13。结论lncRNA?ENST00000420480可作为PA感染的血清学标志物。Objective To investigate the clinical value of the diagnostic marker of long noncoding RNA(LncRNA)ENST00000420480 for Pseudomonas aeruginosa(PA)infection.Methods Human peripheral blood mononuclear cells were separated by density gradient centrifugation and human CD14+monocytesweresorted byimmunomagnetic beads.Mo?DCs were induced by hIL?4 and hGM?CSF.PBS group,LPS group and LPS+3?O?C12?HSL group were divided.Real?time fluorescence quantitative PCR was used to detect the expression of lncRNA?ENST00000420480.Otherwise,a case?control study was performed,Serum samples from 21 patients with clinical PA infections and 32 Physically?sensed healthy human.Serum RNA was extracted and cDNA was obtained by reverse transcription.The expression of lncRNA?ENST00000420480 was detected by real?time quantitative PCR.The receiver operating characteristic curve(ROC)was used to evaluate the diagnostic efficiency of lncRNA?ENST00000420480 for PA infection.Youden(YI)was calculated to determine the critical point of diagnosis of PA infection(cut?off value).Results Compared to the PBS group,LPS lowers the expression level of lncRNA?ENST000000420480 in Mo?DCs.Compared to the LPS group,3?O?C12?HSL upregulated the level of lncRNA?ENST00000420480 in Mo?DCs.The serum level of lncRNA?ENST00000420480 with PA infection group(3.85±4.18)was significantly higher than normal controls(1.30±1.06)(P<0.05).The ROC curve analysis found that the AUC was 0.708.When the YI index is 0.429,the cut?off value is 4.13.Conclusions lncRNA?ENST00000420480 can be used as a serological marker for Pseudomonas aeruginosa infection.

关 键 词：N-3-氧代十二烷酰-L-同型丝氨酸内酯 单核细胞来源树突细胞 长链非编码RNA 

分 类 号：R73[医药卫生—肿瘤]