GPC-3联合CK-19免疫组化检测在肝细胞癌诊断及鉴别诊断中的应用  

Application of GPC-3 Combined with CK-19 Immunohistochemical Detection in Diagnosis and Differential Diagnosis of Hepatocellular Carcinoma

作　　者：刘冰[1] 杨帅[1] 费锡斌 宋健 LIU Bing;YANG Shuai;FEI Xi-bin;SONG Jian(Department of Biochemistry,Baodi District People's Hospital,Tianjin 301800,China;Department of Immunology,Tianjin Central Hospital of Obstetrics and Gynecology,Tianjin 301800,China)

机构地区：[1]天津市宝坻区人民医院生化科,天津301800 [2]天津市中心妇产科医院免疫科,天津301800

出　　处：《医学信息》2019年第23期169-170,共2页Journal of Medical Information

摘　　要：目的研究磷脂酰肌醇蛋白多糖-3(GPC-3)联合细胞角蛋白-19(CK-19)免疫组化检测在肝细胞癌诊断及鉴别诊断中的应用价值。方法选择2017年1月~2019年1月我院通过肝穿刺或外科手术获取的肝癌标本91例。所有标本按照GPC-3及CK-19检测流程处理,比较肝细胞癌(HCC)、肝内胆管细胞癌(ICC)及肝细胞癌合并肝内胆管细胞癌(CHC)标本中GPC-3及CK-19检测阳性率,及不同分化程度HCC的GPC-3及CK-19阳性率。结果HCC与CHC中GPC-3阳性率高于ICC,ICC与CHC中CK-19阳性率高于HCC,差异有统计学意义(P<0.05);中、低分化的HCC标本中GPC-3、CK-19阳性率高于高分化HCC,差异有统计学意义(P<0.05)。结论在肝细胞癌诊断及鉴别诊断中采取GPC-3联合CK-19免疫组化检测,可有效检出HCC,并可与CHC及ICC进行区分,在早期诊断中也具有一定应用价值。Objective To study the application value of phosphatidylinositol-3(GPC-3)combined with cytokeratin-19(CK-19)immunohistochemistry in the diagnosis and differential diagnosis of hepatocellular carcinoma.Methods From January 2017 to January 2019,91 cases of liver cancer specimens obtained by liver puncture or surgery in our hospital were selected.All specimens were processed according to the GPC-3 and CK-19 test procedures to compare hepatocellular carcinoma(HCC),Positive rate of GPC-3 and CK-19 in intrahepatic cholangiocarcinoma(ICC)and hepatocellular carcinoma combined with intrahepatic cholangiocarcinoma(CHC)specimens,and the positive rate of GPC-3 and CK-19 of different degrees of differentiation HCC.Results The positive rate of GPC-3 and CK-19 in HCC and CHC was higher than that in ICC,the difference was statistically significant(P<0.05).The positive rate of GPC-3 in medium and poorly differentiated HCC specimens was higher than that in well differentiated HCC.The positive rate of CK-19 in medium and poorly differentiated HCC specimens was higher than that in well differentiated HCC,the difference was statistically significant(P<0.05).Conclusion GPC-3 combined with CK-19 immunohistochemistry in the diagnosis and differential diagnosis of hepatocellular carcinoma can effectively detect HCC and distinguish it from CHC and ICC,and it has certain application value in early diagnosis.

关 键 词：肝细胞癌 磷脂酰肌醇蛋白多糖-3 细胞角蛋白-19 

分 类 号：R735.7[医药卫生—肿瘤]