Positional cloning of PmCH1357 reveals the origin and allelic variation of the Pm2 gene for powdery mildew resistance in wheat  

作　　者：Fang Chen Haiyan Jia Xiaojun Zhang Linyi Qiao Xin Li Jun Zheng Huijuan Guo Carol Powers Liuling Yan Zhijian Chang 

机构地区：[1]College of Life Science,Shanxi University,Taiyuan 030006,Shanxi,China [2]The Shanxi Province Key Laboratory of Crop Genetics and Gene Improvement,Institute of Crop Science,Shanxi Academy of Agricultural Sciences,Taiyuan 030031,Shanxi,China [3]Department of Plant and Soil Sciences,Oklahoma State University,Stillwater,OK 74078,USA [4]The Applied Plant Genomics Laboratory of Crop Genomics and Bioinformatics Centre,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China [5]Institute of Wheat Research,Shanxi Academy of Agricultural Sciences,Linfen 041000,Shanxi,China

出　　处：《The Crop Journal》2019年第6期771-783,共13页作物学报（英文版）

基　　金：supported by funding from the National Key Research and Development Program of China (2016YFD0102000);Shanxi Provincial Key Platform for Science and Technology Innovation Program (201605D151002);Shanxi Provincial International Cooperation in Science and Technology Program (201803D221018-5);Shanxi Provincial Key Research and Development Project (201803D421020 and 201703D211007);Shanxi Academy of Agricultural Sciences (Project YGG17123)

摘　　要：Powdery mildew, caused by the biotrophic fungus Blumeria graminis f. sp. tritici(Bgt), is a prevalent disease in common wheat(Triticum aestivum L.) and causes serious yield losses worldwide. We used a map-based approach to clone the major broad-spectrum powdery mildew resistance gene Pm CH1357 from wheat breeding line CH1357. Pm CH1357 was mapped to a 526 kb region containing only Traes CS5 D01 G044600. The Traes CS5 D01 G044600 sequence of the susceptibility allele in Taichung 29(TC29) was identical to that in Chinese Spring, whereas the sequence of the resistance allele in CH1357 was identical to Pm2a previously cloned from the germplasm Ulka/*8Cc. The susceptibility allele in TC29 contained a 7 bp deletion in exon 1, resulting in loss of 856 of the 1277 amino acids in the predicted nucleotide-binding domain leucine-rich repeat containing Pm2a protein.Pm CH1357/Pm2a sequence was also isolated from the Chinese wheat landraces and cultivars that were previously reported to possess the resistance gene Pm2b, Pm2c,PmLX66, or PmND399. The Pm CH1357/Pm2a resistance allele was present in 10 of 495 accessions in core germplasm and contemporary cultivars from China and the USA. A newly developed diagnostic marker for the 7 bp In Del in the resistance gene can be used to eliminate the susceptibility allele in wheat breeding programs.Powdery mildew, caused by the biotrophic fungus Blumeria graminis f. sp. tritici(Bgt), is a prevalent disease in common wheat(Triticum aestivum L.) and causes serious yield losses worldwide. We used a map-based approach to clone the major broad-spectrum powdery mildew resistance gene Pm CH1357 from wheat breeding line CH1357. Pm CH1357 was mapped to a 526 kb region containing only Traes CS5 D01 G044600. The Traes CS5 D01 G044600 sequence of the susceptibility allele in Taichung 29(TC29) was identical to that in Chinese Spring, whereas the sequence of the resistance allele in CH1357 was identical to Pm2 a previously cloned from the germplasm Ulka/*8 Cc. The susceptibility allele in TC29 contained a 7 bp deletion in exon 1, resulting in loss of 856 of the 1277 amino acids in the predicted nucleotide-binding domain leucine-rich repeat containing Pm2 a protein.Pm CH1357/Pm2 a sequence was also isolated from the Chinese wheat landraces and cultivars that were previously reported to possess the resistance gene Pm2 b, Pm2 c,Pm LX66, or Pm ND399. The Pm CH1357/Pm2 a resistance allele was present in 10 of 495 accessions in core germplasm and contemporary cultivars from China and the USA. A newly developed diagnostic marker for the 7 bp In Del in the resistance gene can be used to eliminate the susceptibility allele in wheat breeding programs.

关 键 词：Map-based CLONING Natural variation Bgt ISOLATE E09 TRITICUM AESTIVUM Molecular breeding 

分 类 号：S51[农业科学—作物学]