白术多糖对肺癌模型大鼠免疫功能的调节作用及机制研究  

Regulation and mechanism of Atractylodes macrocephala L. polysaccharide on immune function in lung cancer model rats

作　　者：银瑞[1] 王俊钢[1] YIN Rui;WANG Jun-gang(Department of Thoracic Surgery,NanYang Central Hospital,Henan 473000,China)

机构地区：[1]南阳市中心医院胸外科

出　　处：《中国医药生物技术》2019年第6期527-533,共7页Chinese Medicinal Biotechnology

摘　　要：目的研究白术多糖对肺癌模型大鼠免疫相关因子及癌细胞增殖凋亡的影响,探讨其肿瘤抑制机制。方法观察记录不同给药剂量20、50、80 mg/(kg·d)白术多糖对肺癌模型大鼠肺指数、脾脏指数、胸腺指数的影响。全自动血细胞分析仪检测大鼠外周血中白细胞和溶菌酶含量。ELISA法检测大鼠血清中IgG、IgA、IgM水平。流式细胞仪分析T淋巴细胞亚群CD3+、CD4+、CD8+比例。MTT法和Annexin V-FITC/PI双染法分别检测癌细胞增殖和凋亡。结果与模型组相比,白术多糖各剂量组大鼠肺指数均下降(P<0.01),脾脏和胸腺指数上升(P<0.01),大鼠外周血中白细胞和溶菌酶含量升高(P<0.01),且均呈剂量依赖性。ELISA法和流式细胞仪分析结果显示,白术多糖给药组大鼠血清免疫球蛋白IgG、IgA、IgM水平和T淋巴细胞亚群CD3+、CD4+、CD4+/CD8+水平呈剂量依赖性升高(P<0.01)。MTT法和Annexin V-FITC/PI双染法检测结果表明,对比模型组,白术多糖各剂量组肺癌模型大鼠癌细胞增殖率降低(P<0.01),细胞凋亡率增大(P<0.01)。结论白术多糖能够增强肺癌模型大鼠机体免疫功能,抑制癌细胞增殖并诱导凋亡。Objective To explore the effect and mechanism of Atractylodes macrocephala L.polysaccharide on immune factors,cell proliferation and apoptosis in lung cancer model rats.Methods The effect of different doses of Atractylodes macrocephala L.polysaccharide 20,50,80 mg/(kg·d)on lung index,spleen index and thymus index in lung cancer model rats were recorded.White blood cells and lysozyme in peripheral blood of rats were detected by automatic blood cell analyzer.IgG,IgA and IgM in rat serum were detected by ELISA.The ratio of CD3+,CD4+and CD8+was analyzed by flow cytometry.Cell proliferation and apoptosis were detected by MTT and Annexin V-FITC/PI double staining assay,respectively.Results As compared with NS(model group)group,the lung index was decreased(P<0.01)while the spleen,thymus index,leukocytes and lysozyme in rats were increased(P<0.01)of Atractylodes macrocephala L.polysaccharide in each dose group.The result of ELISA and flow cytometry showed that serum immune globulin IgG,IgA,IgM and T lymphocyte subsets CD3+,CD4+,CD4+/CD8+were increased in a dose-dependent manner(P<0.01)in rats treated with Atractylodes macrocephala L.polysaccharide.The results of MTT assay and Annexin V-FITC/PI double staining showed that cell proliferation rate was decreased(P<0.01)and apoptosis rate was increased(P<0.01)of BZ1 group,BZ2 group and BZ3 group.Conclusion Atractylodes macrocephala L.polysaccharide enhances the immunologic function of lung cancer model rats,inhibits cell proliferation and induces apoptosis.

关 键 词：肺肿瘤 免疫球蛋白类 T淋巴细胞 白术多糖 

分 类 号：R73[医药卫生—肿瘤]