长双歧杆菌BB68S体内及体外安全性评价  

Safety Evaluation of Bifidobacteirum Longum BB68S in Vivo and Vitro

作　　者：张晓妹 葛绍阳 桑跃 刘治麟 ZHANG Xiao-mei;GE Shao-yang;SANG Yue;LIU Zhi-lin(College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083;Hebei Technology Advanced Innovation Center for Livestock Food,Langfang 065201;Beijing Heyiyuan Biotechnology Company Limitid,Beijing 100089)

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]河北省畜产食品技术创新中心,廊坊065201 [3]北京和益源生物技术有限公司,北京100089

出　　处：《中国奶牛》2019年第11期43-46,共4页China Dairy Cattle

摘　　要：本研究对长双歧杆菌BB68S进行了体内及体外安全性评价,为菌株的工业化应用提供基础保障。通过体外试验评价了BB68S的抗生素敏感性和生物胺的生成;体内试验评价了BB68S的经口毒性及移位风险。结果表明,菌株BB68S不存在菌株特有的抗生素敏感性,排除了抗生素抗性横向转移的风险;经过在脱羧酶培养基中培养未发现该菌株产生生物胺;灌胃试验和急性经口毒性试验发现,将该菌株分别以108cfu/鼠、109cfu/鼠、1010cfu/鼠剂量饲喂小鼠28d后,小鼠未发生不良反应,在其血液、肾脏、肝脏未发现该菌株。研究未发现菌株BB68S的不安全因素。Bifidobacterium longum BB68S is a potential important probiotic.In this study,the safety of this strain was evaluated in vitro and in vivo,which provided a basic guarantee for the application of the strain.The antibiotic susceptibility and biogenic amine production of BB68S were evaluated by in vitro tests;the oral toxicity and translocation risk of BB68S were evaluated in vivo.The results showed that the strain BB68S did not have the unique antibiotic sensitivity of the strain,and the risk of lateral transfer of antibiotic resistance was ruled out.After the culture in the decarboxylase medium,the strain was not found to produce biogenic amines;the gavage test and the acute oral toxicity test found that after the strain fed to the mice at doses of 108 cfu/mouse,109 cfu/mouse,and 1010 cfu/mouse for 28 days,the mice did not have an adverse reaction,and the strain was not found in the blood,kidney,and liver.The study found no unsafe factors for strain BB68S.

关 键 词：长双歧杆菌BB68S 安全性 抗生素耐性 生物胺 经口毒性 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]