后部巩膜F4/80和CD206阳性巨噬细胞在小鼠形觉剥夺性近视发生发展中的变化  

Changes in F4/80 and CD206 positive macrophages in posterior sclera in the development of form-deprivation myopia of mice

作　　者：李东辉[1] 龙琴[1] 王静怡[1] 陈晨[1] LI Dong-Hui;LONG Qin;WANG Jing-Yi;CHEN Chen(Department of Ophthalmology,Peking Union Medical College Hospital,Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing 100730,China)

机构地区：[1]中国医学科学院,北京协和医学院,北京协和医院眼科

出　　处：《眼科新进展》2019年第12期1116-1119,共4页Recent Advances in Ophthalmology

基　　金：北京市自然科学基金项目(编号:7172173)~~

摘　　要：目的研究后部巩膜F4/80^+和CD206^+巨噬细胞在小鼠形觉剥夺性近视发生发展中的变化趋势。方法将24只(48眼)4周龄C57BL/6小鼠按随机数字表法分别接受形觉剥夺处理和无处理(各12只),研究分为3组:形觉剥夺组(单眼遮盖,n=12),自身对照组(对侧眼无遮盖,n=12),正常对照组(无处理组右眼,n=12)。实验前(0 d)进行屈光度检测;于实验14 d和28 d测量屈光度后分别处死6只小鼠,分离眼球测量眼轴,并分离后部巩膜组织,采用流式细胞术检测后部巩膜组织中F4/80和CD206的表达,观察各指标的动态变化和内在关联。结果实验0 d,形觉剥夺组、自身对照组和正常对照组的屈光度差异均无统计学意义(均为P>0.05)。实验14 d,3组屈光度和眼轴差异均无统计学意义(均为P>0.05);形觉剥夺组后部巩膜F4/80^+巨噬细胞以及F4/80^+、F4/80^+/CD206^+巨噬细胞的百分比均显著高于自身对照组(均为P<0.05)。实验28 d,形觉剥夺组与自身对照组比较,屈光度明显向近视偏移(P=0.001);并伴有眼轴的显著延长(P=0.02);形觉剥夺组后部巩膜F4/80^+巨噬细胞以及F4/80^+/CD206^+巨噬细胞的百分比均显著高于自身对照组(均为P<0.05)。实验28 d,形觉剥夺组小鼠后部巩膜F4/80^+/CD206^+巨噬细胞的百分比显著高于实验14 d时(P=0.02)。实验28 d,形觉剥夺组后部巩膜F4/80^+/CD206^+巨噬细胞的百分比和屈光度呈显著负相关(r=-0.89,P=0.02),和眼轴呈显著正相关(r=0.98,P=0.001)。各时间点自身对照组和正常对照组的各指标差异均无统计学意义(均为P>0.05)。结论 C57BL/6小鼠形觉剥夺性近视的发生和发展中存在后部巩膜组织F4/80^+/CD206^+巨噬细胞的上调,与屈光度和眼轴有相关性,提示F4/80^+/CD206^+巨噬细胞参与近视的发生发展过程。Objective To investigate the dynamic expression levels of F4/80^+ and CD206^+ macrophages in the posterior sclera in mice with form-deprivation myopia.Methods Twenty four C57 BL/6 mice(48 eyes),4-week old,undergoing monocular form-deprivation(12 mice) and untreatment(12 mice),were divided into 3 groups according to random number table:form deprivation group(mice with monocular form-deprivation);self-control group(fellow eyes without form deprivation) and normal control group(no treatment).The refractive diopter of mice was examined before operation,on day 14 and 28.Then 6 mice were sacrificed,and the axial length,the expression levels of F4/80^+ and CD206^+ in the posterior sclera were examined by flow cytometry on day 14 and 28,respectively.The dynamic changes and internal relations of above parameters were investigated.Results There were no significant differences among the 3 groups in terms of refractive diopter(all P>0.05).On day 14,there were no significant differences in refractive diopter and axial length in the three groups(all P>0.05).In the form deprivation group,F4/80^+ macrophages and the percentage of F4/80^+,F4/80^+/CD206^+ macrophages were significantly higher than those of the self-control group(all P<0.05).At 28 d,form deprivation group showed significantly myopic shift(P=0.001) accompanied with elongated axial length(P=0.02) compared with self-control group;in the form deprivation group,F4/80^+ macrophages and the percentage of F4/80^+,F4/80^+/CD206^+ macrophages were significantly higher than those of the self-control group(all P<0.05).In the form deprivation group,F4/80+/CD206+ macrophages in mouse posterior sclera at 14 d was significantly higher compared with that of 28 d(P=0.02).The expression of F4/80^+/CD206^+ was negatively correlated with refractive diopter(r=-0.89,P=0.02) and positively correlated with axial length(r=0.98,P=0.001) on day 28.There were no significant differences in the indexes between the self-control group and the normal control group at each time point(all P>0.05).Con

关 键 词：形觉剥夺 近视 巨噬细胞 巩膜 

分 类 号：R778[医药卫生—眼科]