apelin-APJ系统基因单核苷酸多态性与糖尿病视网膜病变的关系  

Relationship between single nucleotide polymorphism of apelin-APJ system gene and diabetic retinopathy

作　　者：史煜[1] 许译丹 丁波[2] 刘媛[1] 陈力迅 陈春生 SHI Yu;XU Yi-Dan;DING Bo;LIU Yuan;CHEN Li-Xun;CHEN Chun-Sheng(Department of Ophthalmology,Nanjing Hospital Affiliated to Nanjing Medical University(Nanjing First Hospital),Nanjing 210006,Jiangsu Province,China;Department of Endocrinology,Nanjing Hospital Affiliated to Nanjing Medical University(Nanjing First Hospital),Nanjing 210006,Jiangsu Province,China)

机构地区：[1]南京医科大学附属南京医院(南京市第一医院)眼科,江苏省南京市210006 [2]南京医科大学附属南京医院(南京市第一医院)内分泌科,江苏省南京市210006

出　　处：《眼科新进展》2019年第12期1157-1161,共5页Recent Advances in Ophthalmology

基　　金：江苏省科技计划项目(编号:BK20160133)~~

摘　　要：目的研究apelin-APJ系统基因单核苷酸多态性(single nucleotide polymorphism,SNP)与糖尿病视网膜病变(diabetic retinopathy,DR)的关系。方法横断面研究。自2017年7月至2018年7月纳入2型糖尿病患者885例,参照DR国际临床分型标准对患者进行分组,其中DR组346例,非DR(non-DR,NDR)组539例。两组均行眼部检查,计算体质量指数,测量血压,检测空腹血糖,并进行血液生化指标及血脂等实验室检查。采用定量PCR方法获得apelin基因rs3115757、rs56204867、rs3761581和APJ基因rs7119375、rs9943582共5个位点的基因分型,比较不同性别两组间各基因型分布的差异,采用Logistic回归分析apelin和APJ基因5个SNP与DR的关系。结果无论男性还是女性,DR组糖尿病病程均显著长于NDR组(均为P=0.000)。男性apelin基因的3个SNP位点突变等位基因频率在DR组与NDR组间分布差异均有统计学意义(均为P<0.05),APJ基因2个SNP突变位点等位基因频率在DR组与NDR组间分布差异均无统计学意义(均为P>0.05)。女性的5个SNP突变位点等位基因频率在DR组与NDR组间分布差异均无统计学意义(均为P>0.05)。调整年龄、体质量指数、空腹血糖后,Logistic回归分析结果显示,无论男性还是女性,携带rs3115757-C、rs56204867-C、rs3761581-A突变等位基因者患DR的风险均明显高于携带相应野生等位基因者(均为P<0.05)。结论 apelin基因的3个SNP rs3115757、rs56204867、rs3761581与DR的发生有关。Objective To investigate the relationship between single nucleotide polymorphisms of apelin-APJ system and diabetic retinopathy.Methods According to the cross-sectional survey method,885 patients with type 2 diabetes were enrolled from July 2017 to July 2018.The patients were divided into two groups according to the international clinical classification criteria for diabetic retinopathy(DR),including 346 patients in the DR group and 539 patients in the non-DR(NDR) group.Laboratory examinations including eye examination,body mass index(BMI) calculation,blood pressure measurement,fasting blood glucose(FBG) test,blood biochemical indicators and blood lipids were performed in both groups.The genotypes of apelin gene rs3115757,rs56204867,rs3761581 and APJ gene rs7119375 and rs9943582 were obtained by Taqman@MGB probe quantitative PCR method.Differences in genotype distribution and differences in genotype distribution between different sex groups were compared between the two groups.Logistic regression was used to analyze the correlation of DR with 5 SNPs of apelin and APJ genes.Results The duration of diabetes in the DR group was significantly longer than that in the NDR group whether males or females(all P=0.000).The distribution of allele frequencies of the three apelin genes in the males and the NDR groups was statistically significant(all P<0.05).There was no significant difference in the distribution of allele frequencies of two APJ genes between the DR group and the NDR group(all P>0.05).There was no significant difference in the distribution of 5 SNP loci allele frequencies between the DR group and the NDR group(all P>0.05).After adjusting age,BMI,and FBG,logistic regression analysis showed that patients with rs3115757-C,rs56204867-C,and rs3761581-A mutations were more likely to have DR than rs3115757-G,rs56204867-G,rs3761581-T,respectively,regardless of males or females(all P<0.05).Conclusion The three SNPs of apelin gene,rs3115757,rs56204867 and rs3761581,are involved in the occurrence of DR.

关 键 词：APELIN APJ 单核苷酸多态性 糖尿病视网膜病变 

分 类 号：R774.1[医药卫生—眼科]