L型钙通道特异性阻滞剂对食管鳞状细胞癌细胞增殖和细胞周期的影响  

Effect of L-type calcium channel specific blocker on proliferation and cell cycle of esophageal squamous cell carcinoma

作　　者：严冬[1] 程芮[2] YAN Dong;CHENG Rui(Department of Thoracic Surgery,Beijing Children's Hospital,Capital Medical University,Beijing,100045 China;Digestive Disease Center,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京儿童医院胸外科,北京100045 [2]首都医科大学附属北京友谊医院消化中心,北京100050

出　　处：《临床和实验医学杂志》2019年第24期2584-2588,共5页Journal of Clinical and Experimental Medicine

基　　金：国家自然科学基金(编号:81670474)

摘　　要：目的检测L型钙通道特异性阻滞剂硝苯地平、维拉帕米、地尔硫 对食管鳞癌细胞系KYSE410、EC9706增殖和细胞周期的影响。方法选择食管鳞癌细胞系KYSE410、EC9706,将细胞种植于96孔板培养24 h,然后加入不同浓度的L型钙通道特异性阻滞剂硝苯地平、维拉帕米、地尔硫 共同孵育0、24、48、72 h,最后加入3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)并进行吸光度检测。采用流式细胞术方法了解L型钙通道特异性阻滞剂硝苯地平、维拉帕米、地尔硫 对KYSE410、EC9706细胞周期的影响。结果细胞培养结果表明,硝苯地平、维拉帕米和地尔硫 对KYSE410、EC9706细胞株的增殖具有剂量依赖性,组间差异具有明显统计学意义(P <0. 01)。硝苯地平、维拉帕米、地尔硫 对于EC9706细胞增殖抑制具有周期特异性特点,可将EC9706细胞停滞于G0/G1期,其抗增殖作用与细胞周期G1期阻滞有关。结论 L型钙通道特异性阻滞剂硝苯地平、维拉帕米和地尔硫 可通过阻滞L型钙通道从而抑制食管鳞癌细胞株KYSE410、EC9706的增殖,具有浓度依赖性特点,同时将细胞周期停滞于G0/G1期,其抗增殖作用与细胞周期G1期阻滞有关。Objective To investigate whether L-type Ca 2+Channel blocker Nifedipine,Verapamil and Diltiazem can inhibit the proliferation of the human esophageal squamous cancer cell line(EC9706 and KYSE410)and influence the cell cycle.Methods The esophageal squamous cell carcinoma cell lines KYSE410 and EC9706 were selected,and the cells were cultured in 96-well plates for 24 h,and then incubated with different concentrations of L-type calcium channel-specific blockers,nifedipine,verapamil and diltiazem.At 0,24,48,and 72 h,thiazole blue(MTT)was finally added and absorbance was measured..Flow cytometry was used to detect the effects of L-type calcium channel specific blockers nifedipine,verapamil and diltiazem on the cell cycle of KYSE410 and EC9706.Results The results of cell culture showed that nifedipine,verapamil and diltiazem had a dose-dependent proliferation of KYSE410 and EC9706 cell lines,and the difference between the two groups was statistically significant(P<0.01).Nifedipine,verapamil and diltiazem have cycle-specific characteristics for the inhibition of EC9706 cell proliferation,and EC9706 cells could be arrested in G 0/G 1 phase,and its anti-proliferative effect was related to cell cycle G 1 arrest.Conclusion L-type calcium channel-specific blockers,nifedipine,verapamil and diltiazem,inhibit the proliferation of esophageal squamous carcinoma cell lines KYSE410 and EC9706 by blocking L-type calcium channels,and have a concentration-dependent characteristic.The cell cycle is arrested in the G 0/G 1 phase,and its anti-proliferative effect is related to the G1 phase arrest of the cell cycle.

关 键 词：食管鳞状细胞癌 L 型钙通道阻滞剂 细胞增殖 细胞周期 

分 类 号：R73[医药卫生—肿瘤]