不同施肥模式对茶园土壤细菌多样性的影响  

Effects of different fertilization managements on tea garden soil bacterial diversity

作　　者：毛迎新[1] 黄丹娟 王红娟[1] 谭荣荣[1] 陈勋[1] 王友平[2] MAO Ying-xina;HUANG Dan-juana;WANG Hong-juana;TAN Rong-ronga;CHEN Xuna;WANG You-ping(Institute of Fruit and Tea,Hubei Academy of Agricultural Sciences,Wuhan 430064,China;Institute of Plant Protection and Soil Fertilizers/Key Laboratory of Integrated Pest Management Crops in Central China,Ministry of Agriculture,Hubei Academy of Agricultural Sciences,Wuhan 430064,China)

机构地区：[1]湖北省农业科学院果树茶叶研究所,武汉430064 [2]湖北省农业科学院植保土肥研究所/农业部华中作物有害生物综合治理重点实验室,武汉430064

出　　处：《湖北农业科学》2019年第22期65-70,共6页Hubei Agricultural Sciences

基　　金：国家重点研发计划项目(2016YFD0200905);湖北省农业科技创新中心团队项目(2016-620-000-001-032);湖北省第二批现代农业产业技术体系项目

摘　　要：以菜子饼+茶叶配方肥(YSJF1)、脲甲醛缓释肥(YSJF2)、茶叶配方肥(YSJF3)、畜禽粪商品有机肥+茶叶配方肥(YSJF4)、菜子饼+脲甲醛缓释肥(YSJF5)、包膜控释肥(YSJF6)、茶叶复混肥(YSJF7)、不施肥(YSJF8)和习惯施肥(YSJF9)处理后的茶园土壤为研究对象,利用Ion S5TMXL高通量测序技术,以16S rRNA基因为靶标,研究不同施肥模式对茶园土壤细菌数量、多样性和群落结构的影响,分析细菌数量、多样性与土壤理化性质的关联性。在门分类水平上,27个样品共鉴定获得46个类群,其中变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)、放线菌门(Actinobacteria)、绿弯菌门(Chloroflexi)、厚壁菌门(Firmicutes)、奇古菌门(Thaumarchaeota)、拟杆菌门(Bacteroidetes)为优势类群,相对丰度占87.08%~96.76%。不同施肥处理间变形菌门、酸杆菌门、放线菌门、绿弯菌门的相对丰度差异显著。不同施肥处理的ACE指数、Chao1指数、Shannon指数和Simpson指数分别为656.34~962.36、655.79~1 040.41、5.22~6.54和0.925 0~0.969 3。相关性分析和冗余分析结果表明,土壤pH、有机质含量和碱解氮与细菌丰度、α多样性指数和细菌群落结构有一定的相关性,土壤pH是影响细菌群落重要的环境因子。A field trial was conducted in tea plantation, nine different soil samples were collected, including rapeseed cake fertilizer and tea formula fertilizer(YSJF1), urea-formaldehyde slow released fertilizer(YSJF2), tea formula fertilizer(YSJF3), animal manure and tea formula fertilizer(YSJF4), rapeseed cake fertilizer and urea-formaldehyde slow released fertilizer(YSJF5),coated controlled release fertilizer(YSJF6), tea compound fertilizer(YSJF7),no fertilization(YSJF8), and the chemical fertilizer(YSJF9). Using Ion S5 TMXL high-throughput sequencing technology, targeting the 16 S rRNA gene, the effects of different fertilization patterns on the number, diversity and community structure of soil bacteria in tea plantation, and the correlation between the number, diversity and soil physical and chemical properties was analyzed. At the phylum level, forty-six phyla were obtained in all the treatments, among which Proteobacteria, Acidobacteria, Actinobacteria, Chloroflexi, Firmicutes,Thaumarchaeota and Bacteroidetes were the common dominant bacteria, accounting for 87.08% ~96.76% of the total reads.There were significant differences in relative abundance of Proteobacteria, Acidobacteria, Actinobacteria and Chloroflexibetween among different treatments. The index of Ace, Chao1, Shannon and Simpson were 656.34~962.36, 655.79~1 040.41, 5.22~6.54 and 0.925 0 ~0.969 3, respectively. Correlation analysis and redundancy analysis showed that soil pH, organic matter content and alkali-hydrolyzed nitrogen were correlated with bacterial abundance, alpha diversity index and bacterial community structure, and soil pH was an important environmental factor affecting bacterial community.

关 键 词：茶园 施肥模式 细菌菌落多样性 16S RRNA 

分 类 号：S154.3[农业科学—土壤学]