慢加急性乙型肝炎肝衰竭患者中CD163和sCD163的临床表达  

Clinical expression of CD163 and sCD163 in patients with chronic and acute hepatitis B hepatic failure

作　　者：刘国 王博 李劲松[4] 司传平[2] LIU Guo;WANG Bo;LI Jin-song;SI Chuan-ping(School of Medicine and Life Sciences,Shandong Academy of Medical Sciences,Ji′nan University,Shandong Provinec,Jinan 250062,China;Institute of Immunology and Molecular Medicine,Jining Medical College,Shandong Province,Jining 272067,China;College of Forensic Medicine and Medical Laboratory,Jining Medical College,Shandong Province,Jining 272067,China;Jining No.2 People′s Hospital,Shandong Province,Jining 272000,China)

机构地区：[1]济南大学山东省医学科学院医学与生命科学学院,山东济南250062 [2]济宁医学院免疫学与分子医学研究所,山东济宁272067 [3]济宁医学院法医学与医学检验学院,山东济宁272067 [4]山东省济宁市第二人民医院,山东济宁272000

出　　处：《中国当代医药》2019年第33期70-73,共4页China Modern Medicine

摘　　要：目的探讨慢加急性乙型肝炎肝衰竭患者中外周血中CD163和sCD163的临床表达水平。方法选取2015年2月1日~2018年2月10日济南大学山东省医学科学院医学与生命科学学院收治的100例慢加急性乙型肝炎肝衰竭患者作为观察组,选取同期100例健康体检者作为对照组。比较两组的CD163、sCD163、CD163 mRNA表达水平及外周血单个核细胞表面的荧光强度,并分析sCD163与肝损伤关系。结果观察组的CD163[(47.15±16.11)%]、sCD163水平[(33.45±10.25)%]均高于对照组[(16.11±6.23)、(15.11±6.17)%],差异有统计学意义(P<0.05)。观察组的荧光强度[(28.01±8.15)%]高于对照组的[(20.11±1.25)%],差异有统计学意义(P<0.05);观察组的CD163 mRNA表达量高于对照组,差异有统计学意义(P<0.05)。血浆sCD163水平与终末期肝病模型(MELD)评分成正相关关系(r=4.652,P<0.05);与乙型肝炎病毒核酸(HBV-DNA)水平成正相关关系(r=5.521,P<0.05);与人体胆红素数值无相关性(r=6.558,P>0.05)。结论CD163和sCD163的临床表达在慢加急性乙型肝炎肝衰竭患者中具有十分重要的作用,其检测方法简单直接,与疾病进展具有密切的相关性,值得在临床中应用推广。Objective To investigate the clinical expression levels of CD163 and sCD163 in peripheral blood of patients with chronic and acute hepatitis B hepatic failure.Methods One hundred patients with chronic and acute hepatitis B hepatic failure who were admitted to the School of Medicine and Life Sciences of Shandong Academy of Medical Sciences from February 1st,2015 to February 10th,2018,were selected as observation group.One hundred healthy people who underwent medical examinations at the same time were selected as control group.The expression levels of CD163,sCD163 and CD163 mRNA and the fluorescence intensity on the surface of peripheral blood mononuclear cells in the two groups were compared,and the relationship between sCD163 and liver injury was analyzed.Results The levels of CD163([47.15±16.11]%)and scd 163([33.45±10.25]%)in the observation group were higher than those in the control group([16.11±6.23],[15.11±6.17]%),the differences were statistically significant(P<0.05).The fluorescence intensity of the observation group([28.01±8.15]%)was higher than that of the control group([20.11±1.25]%),the difference was statistically significant(P<0.05).The expression of CD163 mRNA in the observation group was higher than that in the control group,and the difference was statistically significant(P<0.05).There was a positive correlation between plasma sCD163 level and mode for end-stage liver disease(MELD)score(r=4.652,P<0.05).There was a positive correlation with the level of hepatitis B virus nucleic acid(HBV-DNA)(r=5.521,P<0.05).There was no correlation with bilirubin(r=6.558,P>0.05).Conclusion The clinical expression of CD163 and sCD163 plays an very important role in patients with chronic and acute hepatitis B hepatic failure,its detection method is simple and direct,has close correlation with disease progression,and is worthy of clinical application and promotion.

关 键 词：慢加急性乙型肝炎肝衰竭 表达差异 免疫组化P16蛋白 

分 类 号：R575.3[医药卫生—消化系统]