发酵液中分离多粘菌素E的生产工艺研究  

Study on the Production Process of Separating Colistin Sulfate E from Fermentation Broth

作　　者：马文艳 潘忠成 王卫富 翁婧 李蒲民 Ma Wenyan;Pan Zhongcheng;Wang Weifu;Weng Jing;Li Pumin(Shaanxi Microbe Biotechnology Co.,Ltd.Weinan,Shaanxi,715500,China;Key Laboratory of Super-Light Materials and Surface Technology,Ministry of Education,College of Material Science and Chemical Engineering,Harbin Engineering University,Harbin,Heilongjiang,150001,China)

机构地区：[1]陕西麦可罗生物科技有限公司,陕西渭南714200 [2]哈尔滨工程大学材料科学与化学工程学院教育部超轻材料与表面技术重点实验室,黑龙江哈尔滨150001

出　　处：《绿色科技》2019年第22期186-189,共4页Journal of Green Science and Technology

基　　金：陕西省科技项目(编号:2018ZKC-173;2019-PT-15);渭南市科技局项目(编号:ZDYF-SFGG-17-ZSG)

摘　　要：主要研究了沉淀法分离提取发酵液中多粘菌素E(Colistin sulfate)的生产工艺。对超滤液热絮凝、吸附脱色、碱性沉淀以及浓缩液二次脱色等工艺参数进行了考察,结果表明:工艺最佳条件:超滤液90~95℃热絮凝5 min后,板换快速冷却,复合脱色处理:加碳量0.1%、亚硫酸盐0.5%~1.0%,沉淀条件:pH值为11.0~12.5、15~30℃、静置1 h,浓缩液脱色添加0.4%~0.8%活性炭,处理0.5 h,最高收率达到90%以上,成品各项指标符合EP生产标准。通过连续稳定性试验对各个参数进行验证,得到了适用于工业化生产的多粘菌素E分离工艺。The production process of separation and extraction of Colistin sulfate from fermentation broth by precipitation method was studied.Thermal flocculation of ultrafiltration,decolorization of activated carbon,alkaline precipitation and secondary decolorization were studied.The optimum conditions of the process:the ultrafiltrate was thermally flocculated at 90-95℃for 5 mins,then cooled and filtered.0.1%activated carbon and 0.5-1.0%sulfites were used for the first decolorization treatment.The precipitation conditions were pH 11.0-2.5 and temperature 15-25℃,and lastly precipitated for 1 hour.The concentrate was decolorized with 0.4-0.8%activated carbon for half an hour.The rate reached over 90%,and the finished products meet the EP production standards.Through the test,each parameter was verified,and the separation process suitable for industrial production was obtained.

关 键 词：硫酸粘杆菌素 沉淀分离法 碱性多肽 絮凝 

分 类 号：TQ923[轻工技术与工程—发酵工程]