海湾扇贝促性腺激素释放激素基因克隆及表达分析  

Cloning and Expression analysis of Gonadotropin-Releasing Hormone Gene in the Bay Scallop

作　　者：李婉茹 张玲玲[1,2] 张美溦 李若佼 李仰平 郭振义[1] 包振民 LI Wan-Ru;ZHANG Ling-Ling;ZHANG Mei-Wei;LI Ruo-Jiao;LI Yang-Ping;GUO Zhen-Yi;BAO Zhen-Min(Key Laboratory of Marine Genetics and Breeding,Ministry of Education,Ocean University of China,Qingdao 266003,China;Laboratory for Marine Fisheries Science and Food Production Processes,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266237,China)

机构地区：[1]中国海洋大学海洋海洋生物遗传学与育种教育部重点实验室,山东青岛266003 [2]青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室,山东青岛266237

出　　处：《中国海洋大学学报（自然科学版）》2020年第2期68-74,共7页Periodical of Ocean University of China

基　　金：国家重点研究发展计划项目(2018YFD0900206)资助~~

摘　　要：促性腺激素释放激素(Gonadotropin Releasing Hormone,GnRH)是脊椎动物下丘脑-垂体-性腺轴的关键信号分子。近年来的研究显示,GnRH也存在于贝类等无脊椎动物,且在雌雄异体性别分化与繁殖中起重要作用,然而雌雄同体贝类GnRH的研究仍处于空白。本文以海湾扇贝为研究对象,利用RACE技术克隆获得GnRH的cDNA全长序列,该基因长1335 bp,编码101个氨基酸,前体包含1个由24个氨基酸组成的信号肽以及1个具有酰胺化修饰的活性肽(QNFHYSNGWQP-amide)。GnRH在脑足神经节和脏神经节中均有表达,且在2015年10月-2016年1月,GnRH在脑足神经节中的表达量显著高于脏神经节(P<0.05);GnRH在两个神经节中的表达均呈现先上升后下降的趋势,峰值出现在11月份,推测可能与性腺发育启动有关。本研究为深入理解GnRH在贝类繁殖调控中的作用奠定了基础。Gonadotropin Releasing Hormone(GnRH) is a key molecule in the hypothalamic-pituitary-gonadal axis of vertebrates. Recent research shows that GnRH also present in molluscs, and it plays a vital role in the sex differentiation and reproduction of dioecism.But its role in hermaphrodites remains unclear. In this study, we cloned the full-length cDNA sequence of GnRH from the bay scallop Argopecten irradians. The full-length cDNA of GnRH is 1 335 bp,encoding 101 amino acids. The predicted protein contains a 24-residue signal peptide, as well as anamidated bioactive peptide(QNFHYSNGWQP-amide). Quantitative real-time PCR was applied to investigate the dynamic expression of GnRH in the parietovisceral ganglion(PVG)and pedal and cerebral ganglia(PGCG) from August 2015 to March 2016. Results showed that GnRH is mainly expressed in the PGCG, and the expression patterns are similar between the two ganglia, with a peak in November, which is possibly related with the initiation of gonadal development. This research will assist in better understanding the role of GnRH in theregulation of mollusc reproduction.

关 键 词：海湾扇贝 促性腺激素释放激素 性腺发育 雌雄同体 性别分化 基因克隆及表达 脏神经节 氨基酸组成 

分 类 号：S917.4[农业科学—水产科学]